β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Beta-Endorphin (bEP)
b-EP
- 编号KSA806Hu11
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, cerebrospinal fluid and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围12.35-1,000pg/mL
- 可适灵敏度4.55pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6804¥ 11340¥ 20412¥ 39690¥ 68040
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对β-内啡肽(bEP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA806Hu21 | β-内啡肽(bEP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA806Hu01 | β-内啡肽(bEP)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA806Hu71 | β-内啡肽(bEP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| CEA806Hu | β-内啡肽(bEP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA806Hu | β-内啡肽(bEP)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA806Hu | β-内啡肽(bEP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA806Hu11 | β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Experimental Dermatology | Endogenous μ-opioid peptides modulate immune response towards malignant melanoma[Publish: RD10279] |
| European Journal of Physiology | Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave [SpringerLink: tv015l3126833315] |
| International Journal of Pharmaceutical Science Invention ISSN(Online) | Effect of Ramadan fasting on endorphin and endocannabinoid level in serum, PBMC and macrophage[Ijpsi: Source] |
| Neurological Sciences | Evaluation of immune parameters in chronic migraine with medication overuse[Pubmed:24867859] |
| Brain Behav. | Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria.[pubmed:28413714] |
| Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties[DOI: 10.1111/jocd.12349] |
| International Journal of Nursing Practice | Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial[Pubmed:29512230] |
| КОРРЕКЦИЯ ЦИТОКИНОВОГО И ГОРМОНАЛЬНОГО ДИСБАЛАНСА ПРИ ЛЕЧЕНИИ СТАБИЛЬНОЙ СТЕНОКАРДИИ НАПРЯЖЕНИЯ[10.25207:1608-6228-2018-25-3-51-55] | |
| Journal of Pediatric Neurosciences | Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria[] |
| Chinese Journal of Physiology | Blood dopamine level enhanced by caffeine in men after treadmill running[Pubmed: 31793465] |
| Free Radical Biology and Medicine | Blue light (λ= 453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in …[Pubmed: 31553937] |
| Journal of Sexual Medicine | Between pleasure and pain: a pilot study on the biological mechanisms associated with BDSM interactions in dominants and submissives[Pubmed: 32044259] |
| JOURNAL OF AFFECTIVE DISORDERS | Pain Sensitivity and Plasma Beta-Endorphin in Adolescent Non-Suicidal Self-Injury[Pubmed: 32961416] |
| EUROPEAN NEUROPSYCHOPHARMACOLOGY | Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot study[Pubmed: 32855024] |
| J Burn Care Res | Efficacy of Acupuncture on Pain Mechanisms, Inflammatory Responses, and Wound Healing in the Acute Phase of Major Burns: An Experimental Study on Rats[34309681] |
| European Journal of Integrative Medicine | Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized …[] |