β-羟丁酸(bHB)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Beta-Hydroxybutyric Acid (bHB)

OHB; BHBA; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid; β-Hydroxybutyric acid

特异性

试剂盒中的抗体对β-羟丁酸(bHB)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志参考文献
EndocrinologyOrphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression[Endocrine:Source]
Chem Commun (Camb)A bioelectronic system for insulin release triggered by ketone body mimicking diabetic ketoacidosis in vitro[PubMed: 25846235]
PLoS OneRegulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine[pubmed:27501393]
Buffalo BulletinAlterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes[]
Journal of Integrative AgricultureTemporal dynamics of nutrient balance, plasma biochemical and immune traits, and liver function in transition dairy cows[]