110KSA003Ge11 | 环磷酸腺苷(cAMP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Pan-species (General,通用) USCN(武汉云克隆科技股份有限公司官方网站)

环磷酸腺苷(cAMP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Cyclic Adenosine Monophosphate (cAMP)

c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate

特异性

试剂盒中的抗体对环磷酸腺苷(cAMP)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Pan-species (General,通用)应用(仅供研究使用,不用于临床诊断!)
CPA003Ge11环磷酸腺苷(cAMP)牛血清白蛋白偶联物Immunogen; SDS-PAGE; WB.
CPA003Ge21环磷酸腺苷(cAMP)卵白蛋白偶联物Immunogen; SDS-PAGE; WB.
CPA003Ge41环磷酸腺苷(cAMP)人血清白蛋白偶联物Immunogen; SDS-PAGE; WB.
PAA003Ge01环磷酸腺苷(cAMP)多克隆抗体ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA003Ge71环磷酸腺苷(cAMP)多克隆抗体(生物素标记)WB; IHC; ICC.
LAA003Ge81环磷酸腺苷(cAMP)多克隆抗体(异硫氰酸荧光素标记)WB; IHC; ICC; IF.
FAA003Ge81抗环磷酸腺苷(cAMP)多克隆抗体(异硫氰酸荧光素标记)FCM
MAA003Ge21环磷酸腺苷(cAMP)单克隆抗体WB; IHC; ICC; IP.
MAA003Ge01环磷酸腺苷(cAMP)单克隆抗体ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA003Ge22环磷酸腺苷(cAMP)单克隆抗体ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA003Ge23环磷酸腺苷(cAMP)单克隆抗体ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA003Ge环磷酸腺苷(cAMP)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA003Ge环磷酸腺苷(cAMP)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA003Ge11环磷酸腺苷(cAMP)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
Int J Clin Exp PatholCold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway[PubMed: 26617802]
Experimental DermatologyLight Emitting Diodes Down‐reCavia (Guinea pig )lates Cathelicidin, Kallikrein, and Toll‐like Receptor 2 Expressions in Keratinocytes and Rosacea‐like Mouse Skin[Pubmed:27315464]
arteriosclerosis thrombosis and vascular biologyP2Y12 Promotes Migration of Vascular Smooth Muscle Cells Through Cofilin Dephosphorylation During Atherogenesis.[pubmed:28062501]
Taylor & FrancisInfluences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation[09540105.2017.1306494]
international journal of biological macromoleculesImmunomodulatory effect of APS and PSP is mediated by Ca2+-cAMP and TLR4/NF-κB signaling pathway in macrophage.[pubmed:27732877]
Front Pharmacol. Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance.[pubmed:28261091]
Allergologia et immunopathologiaThe relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin[pubmed:27955890]
Journal of Enzyme Inhibition and Medicinal ChemistryStructural re-positioning, molecular modelling, oxidative degradation, and biological screening of linagliptin as adenosine 3 receptor (ADORA3) modulators …[Pubmed:29768061]
Frontiers in PhysiologyAstragaloside IV Inhibits Adipose Lipolysis and Reduces Hepatic Glucose Production via Akt Dependent PDE3B Expression in HFD-Fed Mice[Pubmed:29410630]
Nanoscience and Nanotechnology LettersDobutamine Up-Regulates Aquaporin 5 Expression in Septic Pulmonary Edema Model In Vitro via cAMP-PKA/CREB Signaling Pathway[10.1166:nnl.2018.2602]
PLoS OneTuberculous meningitis is associated with higher cerebrospinal HIV-1 viral loads compared to other HIV-1-associated meningitides[Pubmed:29394269]
TheriogenologyDevelopmental competence of buffalo (Bubalus bubalis) denuded oocytes cocultured with cumulus cells: Protective role of cumulus cells[Pubmed:30092373]
Evidence-Based Complementary and Alternative MedicineiTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine[]
EXPERIMENTAL BIOLOGY AND MEDICINECalcimimetic R568 improved cardiac remodeling by classic and novel renin-angiotensin system in spontaneously hypertensive rats[Pubmed: 31159562]
Scientific ReportsDeletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion[Pubmed: 31863073]
REPRODUCTION FERTILITY AND DEVELOPMENTCytoplasm lipids can be modulated through hormone-sensitive lipase and are related to mitochondrial function in porcine IVM oocytes[Pubmed: 32172783]
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGYAstragaloside IV relieves gestational diabetes mellitus in genetic mice through reducing hepatic gluconeogenesis[Pubmed: 32160476]
J Physiol BiochemAltered absorptive function in the gall bladder during cholesterol gallstone formation is associated with abnormal NHE3 complex formation[Pubmed: 32557227]
Biomed PharmacotherAntihypertensive effects of allicin on spontaneously hypertensive rats via vasorelaxation and hydrogen sulfide mechanisms[Pubmed: 32480217]
Legal MedicineDNA microarray analysis of hypothermia-exposed murine lungs for identification of forensic biomarkers[Pubmed: 33161360]
NeuropeptidesAdrenomedullin 2 attenuates LPS-induced inflammation in microglia cells by receptor-mediated cAMP-PKA pathway[33253929]
Oxidative Medicine and Cellular LongevityA Green and Blue Monochromatic Light Combination Therapy Reduces Oxidative Stress and Enhances B-Lymphocyte Proliferation through Promoting ¡­[33828639]
Physiological crosstalk between the Mel1a and Mel1c pathways modulates melatonin-mediated, monochromatic light combination-induced bursa B-lymphocyte?¡­[]
Pflügers Archiv : European journal of physiologyThe role of adrenergic and muscarinic receptors in stress-induced cardiac injury[34245378]
TheranosticsSult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice[34815805]