内皮素1(EDN1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Endothelin 1 (EDN1)
ET1; PPET1; Preproendothelin-1; Big endothelin-1
- 编号KSA482Ra11
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围6.17-500pg/mL
- 可适灵敏度2.71pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对内皮素1(EDN1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA482Ra01 | 内皮素1(EDN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA482Ra01 | 内皮素1(EDN1)活性蛋白 | Cell culture; Activity Assays. |
| CPA482Ra11 | 内皮素1(EDN1)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA482Ra21 | 内皮素1(EDN1)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA482Ra08 | 内皮素1(EDN1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA482Ra01 | 内皮素1(EDN1)多克隆抗体 | WB,ICC/IF |
| LAA482Ra81 | 内皮素1(EDN1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA482Ra71 | 内皮素1(EDN1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA482Ra21 | 内皮素1(EDN1)单克隆抗体 | WB; IHC; ICC; IP. |
| CEA482Ra | 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA482Ra | 内皮素1(EDN1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA482Ra11 | 内皮素1(EDN1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Heart and Circulatory Physiology | B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness[APS: source] |
| PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation[PlosOne: Source] |
| 36 | Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1[PubMed: PMC3695508] |
| Clinics (Sao Paulo). | Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic mice[Pubmed: 24212843] |
| Life Sci. | Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model[Pubmed:25476829] |
| Transpl Int. | One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys[Pubmed:24977654] |
| J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders[Pubmed:25245337] |
| Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation[Pubmed:25580880] |
| Journal of Surgical Research | Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioning[Sciencedirect:S0022480414004909] |
| Pharmaceutical Biology | The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil[PubMed: 26023989] |
| A Periodical of the Hungarian Academy of Sciences | The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patients[PubMed: 26690031] |
| Chemical Research in Toxicology | Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation[PubMed: 25580880] |
| Human & Experimental Toxicology | An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injury[PubMed: 26078283] |
| Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome[science:article] |
| Transplant proc | Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients.[pubmed:27496444] |
| Biomed Journal | Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X.[pubmed:27884380] |
| International Journal of Advanced Research in Biological Sciences | Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactose[pdfcopy:42552] |
| Journal of Drug Metabolisn& Toxicology | Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose[587e5b6508aed3826af45e2d.pdf] |
| Polish Journal of Veterinary Sciences | Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema[pubmed:27760029] |
| Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome[article:S1872-9312(16)30085-0] |
| Journal of Molecular Biomarkers J & Diagnosis | Biochemical and Molecular Markers of Congenital and Senile Cataractous Lenses[DOI: 10.4172/2155-9929.1000318] |
| A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats.[AN=123819331] | |
| Journal of Functional Foods | Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation[10.1016/j.jff.2017.05.033] |
| Radiation Research | Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute …[Pubmed:29809108] |
| Scandinavian Journal of Clinical and Laboratory Investigation | Isolated kidney perfusion: the influence of pulsatile flow[Pubmed:29301417] |
| Endokrynologia Polska | Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension …[Pubmed: 30359461] |
| Journal of Pharmacological Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome[Pubmed: 30342783] |
| Molecular Nutrition & Food Research | Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight …[Pubmed: 30570214] |
| Journal of Cosmetic Dermatology | Whitening effects of cosmetic formulation in the vascular component of skin pigmentation[Pubmed: 31074159] |
| Eurasian Journal of Clinical Sciences | APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan[] |
| Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease[] |
| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats[Pubmed: 32616735] |
| Evidence-Based Complementary and Alternative Medicine | Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats[Pubmed: 33133215] |
| Microvasc Res | Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency[34119534] |
| Biomed Pharmacother | Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti …[34736077] |
| J Ethnopharmacol | Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells[34752899] |
| J Ethnopharmacol | Post-stroke treatment of storax improves long-term outcomes of stroke in rats[34343648] |
| Optical Technologies for Biology and Medicine | Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency[] |