非神经元性烯醇化酶(ENO1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Enolase 1 (ENO1)
NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding
- 编号KSB449Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.114ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6829¥ 11382¥ 20488¥ 39837¥ 68292
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对非神经元性烯醇化酶(NNE)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB449Mu01 | 非神经元性烯醇化酶(ENO1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB449Mu01 | 非神经元性烯醇化酶(ENO1)多克隆抗体 | WB,IHC,ICC/IF |
| LAB449Mu71 | 非神经元性烯醇化酶(ENO1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAB449Mu81 | 非神经元性烯醇化酶(ENO1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| SEB449Mu | 非神经元性烯醇化酶(ENO1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB449Mu | 非神经元性烯醇化酶(ENO1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB449Mu01 | 非神经元性烯醇化酶(ENO1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Analytical Chemistry | Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor[PubMed: 20557064] |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules[Pubmed:27172385] |
| Journal of Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia[Pubmed:29654921] |
| BioMed Research International | The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis[Pubmed: 33062681] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins[] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression[34376212] |
| Clin Proteomics | Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma[Pubmed:35610567] |