脂肪酸结合蛋白 4(FABP4)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Fatty Acid Binding Protein 4 (FABP4)
A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein
- 编号KSB693Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围31.2-2000pg/mL
- 可适灵敏度13.8pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对脂肪细胞型脂肪酸结合蛋白(FABP4)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB693Mu02 | 脂肪酸结合蛋白 4(FABP4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB693Mu01 | 脂肪酸结合蛋白 4(FABP4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB693Mu61 | 脂肪酸结合蛋白 4(FABP4)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB693Mu01 | 脂肪酸结合蛋白 4(FABP4)活性蛋白 | Cell culture; Activity Assays. |
| APB693Mu61 | 脂肪酸结合蛋白 4(FABP4)活性蛋白 | Cell culture; Activity Assays. |
| PAB693Mu06 | 脂肪酸结合蛋白 4(FABP4)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB693Mu02 | 脂肪酸结合蛋白 4(FABP4)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB693Mu01 | 脂肪酸结合蛋白 4(FABP4)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB693Mu71 | 脂肪酸结合蛋白 4(FABP4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAB693Mu81 | 脂肪酸结合蛋白 4(FABP4)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| SEB693Mu | 脂肪酸结合蛋白 4(FABP4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB693Mu | 脂肪酸结合蛋白 4(FABP4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB693Mu01 | 脂肪酸结合蛋白 4(FABP4)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Clinical and Molecular Hepatology | Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASH[PubMed: 23091808] |
| journal of Human Hypertension | Siesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adults[PubMed: 26134622] |
| genetics and molecular research | Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice[PubMed: 25867423] |
| Food Chem | Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes[PubMed: 25952883] |
| Placenta | Increased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsia[Pubmed:26992681] |
| Clin Chim Acta. | Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies.[pubmed:28161274] |
| Oxidative Medicine and Cellular Longevity | Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …[Pubmed:29849871] |
| Nutrition & Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans[Pubmed:29335416] |
| American Journal of Cancer Research | 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4[Pubmed: 31720079] |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …[Pubmed:35212074] |
| cells | The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure[Pubmed:35455943] |
| Bone Research | FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis[Pubmed:35729106] |