脂肪酸合酶(FASN)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Fatty Acid Synthase (FASN)
FAS; OA519; SDR27X1; Short Chain Dehydrogenase/Reductase Family 27X,Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase
- 编号KSC470Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.116ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7560¥ 12600¥ 22680¥ 44100¥ 75600
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特异性
试剂盒中的抗体对脂肪酸合酶(FASN)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC470Hu02 | 脂肪酸合酶(FASN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC470Hu01 | 脂肪酸合酶(FASN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC470Hu02 | 脂肪酸合酶(FASN)多克隆抗体 | WB; IHC; ICC/IF |
| PAC470Hu01 | 脂肪酸合酶(FASN)多克隆抗体 | WB; IHC; ICC; IP. |
| MAC470Hu22 | 脂肪酸合酶(FASN)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC470Hu | 脂肪酸合酶(FASN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC470Hu | 脂肪酸合酶(FASN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSC470Hu01 | 脂肪酸合酶(FASN)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study[Pubmed:25652121] |
| Oncogenesis. | Diet-induced alteration of fatty acid synthase in prostate cancer progression[Pubmed:26878389] |
| Animal Science Journal | Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells[pubmed:28804941] |
| life sciences | Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma[Pubmed: 30914315] |
| Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …[] |
| bmc research notes | The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers[Pubmed: 32698888] |