成纤维细胞生长因子15(FGF15)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Fibroblast Growth Factor 15 (FGF15)
- 编号KSL154Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.070ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 8122¥ 13536¥ 24365¥ 47376¥ 81216
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对成纤维细胞生长因子15(FGF15)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| MEL154Mu | 成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEL154Mu | 成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEL154Mu | 成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEL154Mu | 成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEL154Mu | 成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCL154Mu | 成纤维细胞生长因子15(FGF15)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LML154Mu | 成纤维细胞生长因子15(FGF15)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSL154Mu01 | 成纤维细胞生长因子15(FGF15)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Endocrinology. | Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21[PubMed: 25774553] |
| Inflammatory Bowel Diseases | Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammation[pubmed:27580383] |
| J Lipid Res. | Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis.[pubmed:28130274] |
| BMC Endocrine Disorders | The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance[10.1186/s12902-017-0211-5] |
| Journal of Physiology and Biochemistry | The ileal FGF15/19 to hepatic FGFR4 axis regulates liver regeneration after partial hepatectomy in mice[Pubmed:29468415] |
| Developmental Cell | FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis[Pubmed: 30745141] |
| Endocrinology | Interactions between the gravitostat and the fibroblast growth factor system for the regulation of body weight[] |
| Food & Function | Lactoferrin promotes bile acid metabolism and reduces hepatic cholesterol deposition by inhibiting the farnesoid X receptor (FXR)-mediated enterohepatic axis[Pubmed: 31626262] |
| Journal of Translational Medicine | Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation[Pubmed: 33036625] |
| Chin Med | Si-Wu-Tang ameliorates fibrotic liver injury via modulating intestinal microbiota and bile acid homeostasis[34736501] |
| Int J Biol Sci | Dynamics of the gut-liver axis in rats with varying fibrosis severity[Pubmed:35637968] |