胰高血糖素(GCG)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Glucagon (GCG)
GC; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone
- 编号KSB266Mu11
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围18.52-1500pg/mL
- 可适灵敏度7.22pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对胰高血糖素(GC)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB266Mu01 | 胰高血糖素(GCG)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB266Mu02 | 胰高血糖素(GCG)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB266Mu02 | 胰高血糖素(GCG)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB266Mu01 | 胰高血糖素(GCG)多克隆抗体 | WB; IHC; ICC; IP. |
| CEB266Mu | 胰高血糖素(GCG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB266Mu | 胰高血糖素(GCG)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB266Mu11 | 胰高血糖素(GCG)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Molecular Metabolism | PER2 promotes glucose storage to liver glycogen during feeding and acute fasting by inducing Gys2 PTG and GL expression[PubMed: PMC3773838] |
| Exp Biol Med (Maywood). | Increasing glucagon secretion could antagonize the action of exogenous insulin for glycemic control in streptozocin-induced diabetic rhesus monkeys.[Pubmed: 23760004] |
| European Journal of Endocrinology | Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in human[Eje-online: Source] |
| American Diabetes Association | IL-21 is a major negative regulator of IRF4-dependent lipolysis affecting Tregs in adipose tissue and systemic insulin sensitivity[Pubmed:24430438] |
| Journal of Pharmacy and Pharmacology | Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db?mice, a model of type 2 diabetes mellitus[Pubmed:24611864] |
| European Journal of Pharmacology | Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model[Pubmed:27131827] |
| Appl Physiol Nutr Metab | The glucoreCavia (Guinea pig )latory response to high-intensity aerobic exercise following training in rats with insulin-treated type 1 diabetes mellitus[Pubmed:27175938] |
| American Journal of Translational Research | Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β.[pubmed:28337257] |
| Biomedicine & Pharmacotherapy | Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats.[pubmed:27930980] |
| Diabetologia | Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice[pubmed:28280902] |
| International Journal of Molecular Sciences | Effects of Acute Cold Stress on Liver O-GlcNAcylation and Glycometabolism in Mice[Pubmed: 30231545] |
| European Journal of Pharmacology | Exogenous obestatin decreases beta-cell apoptosis and alfa-cell proliferation in high fat diet and streptozotocin induced type 2 diabetic rats[Pubmed: 30776368] |
| Stress | Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure[Pubmed: 30821572] |
| international journal of molecular sciences | Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice[Pubmed: 30909542] |
| Diabetes-Metabolism Research and Reviews | Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cell Transplantation inhibits beta‐cells apoptosis via mediating the ASK1/JNK/BAX pathway in [Pubmed: 31411368] |
| CELL DISCOVERY | In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals[Pubmed: 32284878] |
| endocrine journal | Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose …[Pubmed: 32249244] |
| Frontiers in Endocrinology | RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis[Pubmed: 32328034] |
| Molecular Medicine Reports | Acarbose ameliorates spontaneous type‑2 diabetes in db/db mice by inhibiting PDX‑1 methylation[Pubmed: 33236139] |
| CHEMICO-BIOLOGICAL INTERACTIONS | Modulatory role of imatinib mesylate on pancreatic β-cells' secretory functions in an STZ rat model of diabetes mellitus[Pubmed: 32710900] |
| Mol Cell Endocrinol | Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells[34391847] |