生长分化因子5(GDF5)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Growth Differentiation Factor 5 (GDF5)

CDMP1; LAP4; SYNS2; BMP14; Radotermin; Cartilage-Derived Morphogenetic Protein-1; Bone morphogenetic protein 14; Lipopolysaccharide-associated protein 4

特异性

试剂盒中的抗体对生长分化因子5(GDF5)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Homo sapiens (Human,人)应用(仅供研究使用,不用于临床诊断!)
RPC110Hu01生长分化因子5(GDF5)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
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SEC110Hu生长分化因子5(GDF5)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
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AEC110Hu抗生长分化因子5抗体(Anti-GDF5)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antibody Detection.
SCC110Hu生长分化因子5(GDF5)检测试剂盒(化学发光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMC110Hu生长分化因子5(GDF5)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSC110Hu01生长分化因子5(GDF5)检测试剂盒DIY材料(酶联免疫吸附试验法)自己动手制作ELISA试剂盒的主要材料

参考文献

杂志参考文献
Carbohydrate PolymersZrO2 surface chemically coated with hyaluronic acid hydrogel loading GDF-5 for osteogenesis in dentistry[PubMed: 23218279]
BonePhoto-cured hyaluronic acid-based hydrogels containing growth and differentiation factor 5 (GDF-5) for bone tissue regeneration[ScienceDirect: S875632821300481X]
J Nanosci NanotechnolIn Vitro Osteogenic Differentiation Enhanced by Zirconia Coated with Nano-Layered Growth and Differentiation Factor-5[Pubmed:27398455]
Macromolecular ResearchOsteoblastic Differentiation of Functionalized Biphasic Hydroxyapatite and b-Tricalcium Phosphate with Recombinant Human Growth and Differentiation (rhGDF-5)[]
Pharmaceutics3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells[34575523]