27kDa热休克蛋白(Hsp27)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Heat Shock Protein 27 (Hsp27)
HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
- 编号KSA693Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.78-50ng/mL
- 可适灵敏度0.32ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6998¥ 11664¥ 20995¥ 40824¥ 69984
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对27kDa热休克蛋白(HSPB1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA693Mu02 | 27kDa热休克蛋白(Hsp27)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA693Mu01 | 27kDa热休克蛋白(Hsp27)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA693Mu01 | 27kDa热休克蛋白(Hsp27)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA693Mu02 | 27kDa热休克蛋白(Hsp27)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA693Mu | 27kDa热休克蛋白(Hsp27)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA693Mu | 27kDa热休克蛋白(Hsp27)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA693Mu01 | 27kDa热休克蛋白(Hsp27)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells[PubMed: 26467738] |
| Nature Communications | HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis[Pubmed:29650953] |
| Advances in Experimental Medicine and Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease[Pubmed:29429028] |
| Diabetes Research and Clinical Practice | The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity[Pubmed: 31102684] |
| Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients[] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort[Pubmed: 32210109] |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive …[Pubmed:35687771] |
| Beilstein Archives | Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation[] |