组胺(HA)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Histamine (HA)

Histamin

  • 组胺(HA)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 组胺(HA)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对Histamine (HA)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志参考文献
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Int J Clin Exp PatholEffects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism[files:ijcep0019305.pdf]
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Journal of Cosmet Dermatol.Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties[DOI: 10.1111/jocd.12349]
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AllergyArtemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial[Pubmed: 32030780]
MOLECULAR IMMUNOLOGYAnimal models for analysis of hypersensitivity reactions to Shuanghuanglian injection[Pubmed: 32302806]
bioscience reportsProtective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat[Pubmed: 32395767]
Nature CommunicationsA vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy[Pubmed: 32332752]
CCR3‑shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells[Pubmed: 32742345]
ANALYTICA CHIMICA ACTAMultiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of …[Pubmed: 32892931]
Annals of Tropical Medicine & Public Health Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq[]
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