胰岛素样生长因子结合蛋白1(IGFBP1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 1 (IGFBP1)

IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein

特异性

试剂盒中的抗体对胰岛素样生长因子结合蛋白1(IGFBP1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Bos taurus; Bovine (Cattle,牛)应用(仅供研究使用,不用于临床诊断!)
RPA052Bo01胰岛素样生长因子结合蛋白1(IGFBP1)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA052Bo01胰岛素样生长因子结合蛋白1(IGFBP1)多克隆抗体WB; IHC; ICC; IP.
MAA052Bo21胰岛素样生长因子结合蛋白1(IGFBP1)单克隆抗体WB; IHC; ICC; IP.
SEA052Bo胰岛素样生长因子结合蛋白1(IGFBP1)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA052Bo胰岛素样生长因子结合蛋白1(IGFBP1)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA052Bo01胰岛素样生长因子结合蛋白1(IGFBP1)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
EndocrinologyLack of Dietary Carbohydrates Induces Hepatic Growth Hormone (GH) Resistance in Rats[Endo: source]
EndocrinologySerum IGF-I Is Not a Reliable Pharmacodynamic Marker of Exogenous Growth Hormone Activity in Mice[Endo: source]
Animal.Effects of a dietary crude fibre concentrate on growth in weaned piglets.[pubmed:28318464]
Stem Cell Research & TherapyDifferentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells[pubmed:29096715]