白介素31(IL31)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Interleukin 31 (IL31)
- 编号KSB179Ra01
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围15.6-1,000pg/mL
- 可适灵敏度6.7pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对白介素31(IL31)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| EPB179Ra61 | 白介素31(IL31)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB179Ra01 | 白介素31(IL31)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB179Ra01 | 白介素31(IL31)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB179Ra81 | 白介素31(IL31)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB179Ra71 | 白介素31(IL31)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB179Ra21 | 白介素31(IL31)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB179Ra | 白介素31(IL31)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB179Ra | 白介素31(IL31)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB179Ra01 | 白介素31(IL31)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| allergy and clinical immunology | Increased serum levels of Interleukin-31 could modulate inflammatory processes in multiple sclerosis[Siaic-Review:Source] |
| BMC Immunol | Increased levels of interleukin 31 (IL-31) in osteoporosis[PubMed: 2644965] |
| Sci Rep. | Interleukin-31 expression and relation to disease severity in human asthma[pmc:PMC4783779] |
| Exp Dermatol. | Immediate-type allergic and protease-mediated reactions are involved in scratching behaviour induced by topical application of Dermatophagoides farinae extract in NC/Nga mice.[pubmed:28191683] |
| Nat Commun. | The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction.[pubmed:28067314] |
| Experimental Dermatology | Immediate-type allergic and protease-media ted reactions are involved in scratching behavior induced by topical application of Dermatophagoides farinae extract in NC/Nga mice[doi:10.1111] |
| アトピー性皮膚炎の痒みの発症機序に関する研究[] | |
| Indian Journal of Public Health Research & Development | Determination the Role of Interleukin 31 (IL-31) Levels in Three Real Allergic Diseases (Asthma, Rhinitis, Urticaria)[] |