亲核素α2(KPNa2)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Karyopherin Alpha 2 (KPNa2)

IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha

  • 亲核素α2(KPNa2)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 亲核素α2(KPNa2)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对亲核素α2(KPNa2)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志参考文献
Oncol Rep.?KPNA2 is a promising biomarker candidate for esophageal squamous cell carcinoma and correlates with cell proliferation[Pubmed:25109899]
Tumor BiologyKPNA2 is a potential diagnostic serum biomarker for epithelial ovarian cancer and correlates with poor prognosis[pubmed:28651492]
Head & NeckAssociation of overexpressed karyopherin alpha 2 with poor survival and its contribution to interleukin‐1β‐induced matrix metalloproteinase expression in oral cancer[Pubmed:29542209]
International Journal of CancerNuclear transport proteins are secreted by cancer cells and identified as potential novel cancer biomarkers[34591985]