脂蛋白脂酶(LPL)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Lipoprotein lipase (LPL)

LIPD; Lipase, Lipoprotein

  • 脂蛋白脂酶(LPL)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 脂蛋白脂酶(LPL)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对脂蛋白脂酶(LIPD)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Rattus norvegicus (Rat,大鼠)应用(仅供研究使用,不用于临床诊断!)
RPA386Ra01脂蛋白脂酶(LPL)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA386Ra01脂蛋白脂酶(LPL)多克隆抗体WB; IHC; ICC/IF
MAA386Ra21脂蛋白脂酶(LPL)单克隆抗体WB; IHC; ICC; IP.
SEA386Ra脂蛋白脂酶(LPL)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA386Ra脂蛋白脂酶(LPL)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA386Ra01脂蛋白脂酶(LPL)检测试剂盒DIY材料(酶联免疫吸附试验法)自己动手制作ELISA试剂盒的主要材料
PGA386Ra01脂蛋白脂酶(LPL)引物对PCR

参考文献

杂志参考文献
DiabetesSeverity of Diabetes Governs Vascular Lipoprotein Lipase by Affecting Enzyme Dimerization and Disassembly[PubMed: 21646389]
The Korean Journal of Internal MedicineClinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation[Pubmed:27243230]
The Journal of Lipid ResearchReCavia (Guinea pig )lation of Plasma Lipid Homeostasis by Hepatic Lipoprotein Lipase in Adult Mice[Pubmed:27234787]
Journal of Functional FoodsPolysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet[10.1016/j.jff.2017.07.020]
Biotechnology ProgressMass Spectrometric Evaluation of Upstream and Downstream Process Influences on Host Cell Protein Patterns in Biopharmaceutical Products[Pubmed: 30767403]
arteriosclerosis thrombosis and vascular biologyIntegrin β3 Deficiency Results in Hypertriglyceridemia Via Disrupting LPL (Lipoprotein Lipase) Secretion[Pubmed: 32237906]
Clinical Nutrition ESPENAbdominal fat distribution modulates the metabolic effects of exogenous ketones in individuals with new-onset prediabetes after acute pancreatitis: Results from a randomized placebo-controlled trial[34024503]
AntioxidantsBio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory …[Pubmed:35204215]