武汉云克隆科技股份有限公司

巨噬细胞移动抑制因子(MIF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Macrophage Migration Inhibitory Factor (MIF)

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

  • 巨噬细胞移动抑制因子(MIF)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 巨噬细胞移动抑制因子(MIF)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对巨噬细胞移动抑制因子(MIF)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
APA698Hu02巨噬细胞移动抑制因子(MIF)活性蛋白Cell culture; Activity Assays.
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RPA698Hu02巨噬细胞移动抑制因子(MIF)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA698Hu01巨噬细胞移动抑制因子(MIF)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA698Hu06巨噬细胞移动抑制因子(MIF)多克隆抗体WB
PAA698Hu01巨噬细胞移动抑制因子(MIF)多克隆抗体WB; IHC
PAA698Hu02巨噬细胞移动抑制因子(MIF)多克隆抗体WB; IHC
MAA698Hu22巨噬细胞移动抑制因子(MIF)单克隆抗体WB; IHC; ICC; IP.
SEA698Hu巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA698Hu巨噬细胞移动抑制因子(MIF)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA698Hu01巨噬细胞移动抑制因子(MIF)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
Kidney International Targeted reduction of advanced glycation improves renal function in obesity[PubMed: 21412218]
The Journal of ImmunologyMacrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut[Jimmunol: 5673]
The Journal of EndocrinologyInvolvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease[PubMed: PMC3757527]
The Journal of ImmunologyRole of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice[PubMed: PMC3466372]
DiabetologiaDeletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes[Pubmed:24957662]
BioMed Research International The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder[PubMed: 25705692]
DiabetologiaCombined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease.[pubmed:28160092]
Life SciencesProtective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis [pubmed:28919396]
Scientific ReportsCytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke[Pubmed:29335619]
Ocular Immunology and InflammationImmune Response and Mechanisms of IFN-γ in Administration for Keratomycosis[Pubmed: 30307777]
International Journal of Immunopathology and PharmacologyEffect of voluntary running on expression of myokines in brains of rats with depression[Pubmed: 30834799]
Biomarkers in Heart Failure and Associated DiseasesBiomarkers in Heart Failure and Associated Diseases[]
Science China-Life SciencesAutophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation[Pubmed: 31209799]
Folia MorphologicaBeneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression[Pubmed: 31802473]
Biomed PharmacotherDeletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis[Pubmed: 32062385]
Sci RepAssociation between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population[33850223]
Commun BiolMacrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes[Pubmed:35115654]