基质金属蛋白酶10(MMP10)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Matrix Metalloproteinase 10 (MMP10)

STMY2; SL2; Stromelysin 2; Transin-2

  • 基质金属蛋白酶10(MMP10)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 基质金属蛋白酶10(MMP10)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对基质金属蛋白酶10(MMP10)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志参考文献
Journal of Cellular and Molecular MedicineType-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage heart failure patients: relationship between MMP-10 and LV remodelling[Wiley: source]
PLoS One.Matrix Metalloproteinase-10 Is Required for Lung Cancer Stem Cell Maintenance, Tumor Initiation and Metastatic Potential[PubMed: PMC3335833]
PLoS ONEA Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source]
Thromb Haemost.Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke[Pubmed:24671655]
Brain, Behavior, and ImmunityPlasma inflammatory biomarkers for Huntington’s disease patients and mouse model [Pubmed:25266150]
Biomedical MaterialsIn vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study[Pubmed:25634132]
Brain, Behavior, and ImmunityPlasma inflammatory biomarkers for Huntington’s disease patients and mouse model[PubMed: 25266150]