武汉云克隆科技股份有限公司

中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)

ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein

  • 中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 中脑星形胶质细胞来源神经营养因子(MANF)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对中脑星形胶质细胞来源神经营养因子(MANF)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
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参考文献

杂志参考文献
PLoS One.Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing[PubMed: PMC3378547]
Endocr J.Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance.[pubmed:28216543]
Journal of Steroid Biochemistry and Molecular BiologyDepressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling[pubmed:28263910]
Endocrine JournalCirculating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance[10.1507:endocrj.EJ16-0472]
Nature MetabolismMANF regulates metabolic and immune homeostasis in ageing and protects against liver damage[]
J Exp MedFeeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway[33856409]
J Inflamm ResThe Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia …[34234505]