粘蛋白1(MUC1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Mucin 1 (MUC1)
CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; CA153; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin
- 编号KSA413Ra01
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.135ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对粘蛋白1(MUC1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA413Ra01 | 粘蛋白1(MUC1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA413Ra02 | 粘蛋白1(MUC1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA413Ra01 | 粘蛋白1(MUC1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA413Ra02 | 粘蛋白1(MUC1)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA413Ra21 | 粘蛋白1(MUC1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA413Ra | 粘蛋白1(MUC1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA413Ra | 粘蛋白1(MUC1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA413Ra01 | 粘蛋白1(MUC1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Annals of surgical oncology | Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.[PubMed: 20717734] |
| J Hum Lact | Host Defense Proteins in Breast Milk and Neonatal Yeast Colonization[PubMed: 26116637] |
| Breast | Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator[PubMed: 26446295] |
| PLoS One. | The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital Fluids[Pubmed:27182834] |
| Int J Clin Exp Med | The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai[files:ijcem0020163.pdf] |
| Talanta | Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification.[pubmed:28107977] |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489] |
| Bio-protocol | Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA[10.21769:BioProtoc.2879] |
| Biosensors and Bioelectronics | Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review[] |
| Journal of Electroanalytical Chemistry | A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample[] |