肌肉生长抑制素(MSTN)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Myostatin (MSTN)
GDF8; Growth Differentiation Factor 8
- 编号KSB653Mu11
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围617.3-50,000pg/mL
- 可适灵敏度274.5pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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特异性
试剂盒中的抗体对肌肉生长抑制素(MSTN)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB653Mu02 | 肌肉生长抑制素(MSTN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB653Mu01 | 肌肉生长抑制素(MSTN)活性蛋白 | Cell culture; Activity Assays. |
| RPB653Mu01 | 肌肉生长抑制素(MSTN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPB653Mu21 | 肌肉生长抑制素(MSTN)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAB653Mu08 | 肌肉生长抑制素(MSTN)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB653Mu02 | 肌肉生长抑制素(MSTN)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB653Mu01 | 肌肉生长抑制素(MSTN)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB653Mu71 | 肌肉生长抑制素(MSTN)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEB653Mu | 肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB653Mu | 肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB653Mu | 肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB653Mu11 | 肌肉生长抑制素(MSTN)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Clinical Medicine and Diagnostics | Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats[Sapub:Source] |
| Nutr Metab (Lond) | Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice[PubMed: 26539241] |
| International Journal of Cancer | Tumor inoculation site affects the development of cancer cachexia and muscle wasting[PubMed: 26016447] |
| Connect Tissue Res | Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts[PubMed: 25748814] |
| PLoS One | Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease[Pubmed:26998756] |
| Calcif Tissue Int. | The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats[Pubmed:27272029] |
| Archives of medical research | Irisin and Myostatin Levels in Patients with Graves' Disease.[pubmed:27986127] |
| Calcified Tissue International | Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats[pubmed:29242963] |
| Scientific Reports | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis.[pubmed:28740214] |
| Journal of Animal Science | Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs[Pubmed: 30203098] |
| European Journal of Internal Medicine | Predictive value of serum myostatin for the severity and clinical outcome of heart failure[Pubmed: 31056368] |
| J Cell Physiol | Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats[Pubmed: 31960436] |
| NEPHROLOGY DIALYSIS TRANSPLANTATION | Myostatin and muscle atrophy during chronic kidney disease[Pubmed: 32974666] |
| Oxid Med Cell Longev | Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance …[34868459] |