降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Procalcitonin (PCT)

Pro-Calcitonin

  • 降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对降钙素原(PCT)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Chicken (Gallus,鸡)应用(仅供研究使用,不用于临床诊断!)
RPA689Ga01降钙素原(PCT)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA689Ga01降钙素原(PCT)多克隆抗体WB; IHC; ICC; IP.
KSA689Ga01降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法)自己动手制作ELISA试剂盒的主要材料

参考文献

杂志参考文献
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International Journal of Radiation Oncology ? Biology ? Physics Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice[PubMed: 20637981]
Ulus Travma Acil Cerrahi DergDiagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia[PubMed: 21935794]
Egyptian Journal of BronchologySerum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children[Essbronchology: Source]
University of Waikato The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis[Waikato:Source]
Egyptian Journal of Medical MicrobiologyEvaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis[Ejmm:Source]
Nigerian Medical JournalEfficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis[Pubmed:25657488]
Wiley online libraryThe use and value of procalcitonin in solid organ transplantation[PubMed: 25996831]
Balkan Med JDiagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia[PubMed: 26185718]
The Journal of Clinical Endocrinology & MetabolismMacrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay[PubMed: 26647152]
Journal of Human HypertensionProcalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension[PubMed: 26040439]
Medical Science MonitorVariation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection.[Pubmed:26772168]
International ImmunopharmacologyConsistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery[Pubmed:26802602]
Pancreatology.Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis.[Pubmed:26804005]
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The Clinical Respiratory JournalChanges in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome[pubmed:28960823]
SHOCKINTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION[pubmed:27984536]
Cellular Physiology and BiochemistryHepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure[Pubmed:29913443]
Archives of Medical ScienceInflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase[Pubmed:29593801]
Journal of Thoracic DiseaseExpression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung[Pubmed:29707313]
Asian Journal of PsychiatryProcalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients[Pubmed:29723719]
European Review for Medical and Pharmacological SciencesThe predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia.[Pubmed: 31298341]
MedicinaModestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection[Pubmed: 33080909]
MEDIATORS OF INFLAMMATIONReevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase[Pubmed: 32410851]
Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes[]
Journal of Immunology ResearchMoesin Is a Novel Biomarker of Endothelial Injury in Sepsis[33628853]
Journal of Medical BiochemistryCirculating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer[33776564]
Surg EndoscOriginal contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesity[Pubmed:35044516]
Bone ResProcalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment[Pubmed:35087025]