武汉云克隆科技股份有限公司

Ⅱ型前胶原羧基端原肽(PIICP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Procollagen II C-Terminal Propeptide (PIICP)

P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

  • Ⅱ型前胶原羧基端原肽(PIICP)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Ⅱ型前胶原羧基端原肽(PIICP)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对Ⅱ型前胶原羧基端原肽(PIICP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

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参考文献

杂志参考文献
Experimental and Therapeutic MedicineEvaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: A …[10.3892]
Functional Foods in Health & DiseaseEvaluation of the effect of administering N-acetyl-glucosamine-containing green tea supplement on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: a randomized double-blind placebo-controlled clinical study[309]
EXPERIMENTAL AND THERAPEUTIC MEDICINEEffect of N-acetylglucosamine administration on cartilage metabolism and safety in healthy subjects without symptoms of arthritis: A case report[pubmed:28413518]
Experimental and Therapeutic MedicineEvaluation of the effect of salmon nasal proteoglycan on biomarkers for cartilage metabolism in individuals with knee joint discomfort: A randomized double‑blind placebo‑controlled clinical study[etm:14]
Experimental and Therapeutic MedicineEvaluation of the efficacy of Ajuga decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double‑blind, placebo‑controlled study[10.3892/etm.2017.5064]
EXPERIMENTAL AND THERAPEUTIC MEDICINEEvaluation of the chondroprotective action of N‑acetylglucosamine in a rat experimental osteoarthritis [pubmed:28912864]
Functional Foods In Health And DiseaseEvaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals: a randomized double-blind placebo-controlled clinical study[view/366]
SpineNo effects of hyperosmolar culture medium on tissue regeneration by human degenerated nucleus pulposus cells despite upregulation extracellular matrix genes[fulltext:2018/03010]
Advances in Clinical and Experimental MedicineFibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with …[68362.pdf]
Functional Food Researchサケ鼻軟骨由来プロテオグリカン摂取による関節保護効果[]
SpineNo Effects of Hyperosmolar Culture Medium on Tissue Regeneration by Human Degenerated[Pubmed: 25856264]
Applied Physiology Nutrition and MetabolismEffectiveness of collagen supplementation on pain scores in healthy individuals with self-reported knee pain; A randomized controlled trial[Pubmed: 31990581]
International Journal of Molecular SciencesBone Morphogenetic Proteins for Nucleus Pulposus Regeneration[Pubmed: 32295299]
BiomoleculesConcerted Actions by PIICP, CTXII, and TNF-¦Á in Patients with Juvenile Idiopathic Arthritis[33924892]