丝甘蛋白聚糖(SRGN)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Serglycin (SRGN)
PRG; PPG; PRG1; proteoglycan 1, secretory granule
- 编号KSC869Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.059ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7110¥ 11850¥ 21330¥ 41475¥ 71100
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对丝甘蛋白聚糖(SRGN)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC869Mu01 | 丝甘蛋白聚糖(SRGN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC869Mu01 | 丝甘蛋白聚糖(SRGN)多克隆抗体 | WB; IHC; ICC; IP. |
| LAC869Mu81 | 丝甘蛋白聚糖(SRGN)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAC869Mu71 | 丝甘蛋白聚糖(SRGN)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEC869Mu | 丝甘蛋白聚糖(SRGN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC869Mu | 丝甘蛋白聚糖(SRGN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSC869Mu01 | 丝甘蛋白聚糖(SRGN)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Irish Journal of Medical Science | The role of increased synovial fluid A disintegrin and metalloproteinase with thrombospondin motifs4 and serglycin levels in osteoarthritis[Pubmed: 30536194] |
| MOLECULAR AND CELLULAR BIOCHEMISTRY | SRGN, a new identified shear-stress-responsive gene in endothelial cells[Pubmed: 32712749] |
| Theranostics | Significance of serglycin and its binding partners in autocrine promotion of metastasis in esophageal cancer[33456569] |
| CELLULAR ONCOLOGY | Cancer©\associated fibroblasts secrete hypoxia©\induced serglycin to promote head and neck squamous cell carcinoma tumor cell growth in vitro and in vivo by ¡[33651283] |
| Cell Death Dis | Serglycin induces osteoclastogenesis and promotes tumor growth in giant cell tumor of bone[34556636] |