沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Sirtuin 1 (SIRT1)
SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment
- 编号KSE912Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.067ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7776¥ 12960¥ 23328¥ 45360¥ 77760
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对沉默调节蛋白1(SIRT1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPE912Mu01 | 沉默调节蛋白1(SIRT1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPE912Mu02 | 沉默调节蛋白1(SIRT1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE912Mu01 | 沉默调节蛋白1(SIRT1)多克隆抗体 | WB |
| PAE912Mu02 | 沉默调节蛋白1(SIRT1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAE912Mu71 | 沉默调节蛋白1(SIRT1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEE912Mu | 沉默调节蛋白1(SIRT1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME912Mu | 沉默调节蛋白1(SIRT1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSE912Mu01 | 沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| PLoS One. | SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases[Plos: Source] |
| PLOS ONE | A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats[Pubmed:25501750] |
| Journal of Alzheimer's Disease | Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment[Pubmed:25589731] |
| Respirology | Increases in peripheral SIRT1: A new biological characteristic of asthma[PubMed: 26040995] |
| PLOS ONE | A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly:[PubMed: 25785999] |
| Endocrine | Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease.[pubmed:27522360] |
| Naunyn Schmiedebergs Arch Pharmacol | The potential curative effect of rebamipide in hepatic ischemia/reperfusion injury[pubmed:28361247] |
| International journal of cardiology | A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease[pubmed:28043667] |
| International Journal of Cardiology | Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial[pubmed:28029409] |
| Hepatology International | Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population[pubmed:29189974] |
| Toxicology and Applied Pharmacology | Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1)[pubmed:28974454] |
| Chemico-biological Interactions | Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction[pubmed:28919305] |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after …[Pubmed:30037068] |
| Experimental Gerontology | Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia[Pubmed:30071285] |
| Kidney and Blood Pressure Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy[Pubmed: 30504713] |
| Journal of International Medical Research | Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical study[Pubmed: 30556451] |
| BioMed Research International | Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic …[] |
| 学位论文 | Influência do tratamento da doença periodontal na sirtuína 1 e na lectina ligadora de manose em indivíduos com doença arterial coronária[] |
| Journal of Physics D: Applied Physics | Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility[] |
| Journal of Geriatric Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors[Pubmed: 30952517] |
| Biomed Research International | Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of …[Pubmed: 30956976] |
| Journal of Diabetes and its Complications | Vasohibin-1 in T2DM with different UACR[] |
| Clinical Interventions in Aging | The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance Hemodialysis[Pubmed: 32214805] |
| Compr Psychiatry | Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factor[Pubmed: 33045669] |
| Сибирский федеральный университет | Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте[] |
| Journal of Cereal Science | Dietary buckwheat enhances sirtuin1 without calorie restriction[] |
| Clin Interv Aging | Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study[33447019] |
| Antioxidants | The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of?¡[33396247] |
| Int Urol Nephrol | Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease[33864594] |
| Neurochem Int | Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ¡[34052296] |
| Toxicol Appl Pharmacol | Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-IкBα/Ubc9 and SIRT-1/miR-22 trajectories[34174262] |
| Food Chem | Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats[34763933] |
| Life Sci | Novel mechanistic insights towards the repositioning of alogliptin in Parkinson's disease[34774622] |
| Int J Mol Sci | Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient Rats[Pubmed:35163435] |
| J Clin Med | Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter?[Pubmed:35683374] |
| Cell & Bioscience | OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress[] |