P物质(SP)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Substance P (SP)
- 编号KSA393Ra11
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围24.69-2,000pg/mL
- 可适灵敏度
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对P物质(SP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA393Ra22 | P物质(SP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA393Ra01 | P物质(SP)多克隆抗体 | WB; IHC; ICC; IP. |
| CEA393Ra | P物质(SP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA393Ra | P物质(SP)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA393Ra | P物质(SP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA393Ra11 | P物质(SP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Veterinary Internal Medicine | A Potential Role for Substance P and Interleukin-6 in the Cerebrospinal Fluid of Cavalier King Charles Spaniels with Neuropathic Pain[Pubmed: 23659719] |
| Pharmacol Res | Oral curcumin has anti-arthritic efficacy through somatostatin generation[PubMed: 25836921] |
| Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation[10.1007/s13765-017-0327-3] |
| CNS Neuroscience & Therapeutics | Direct activation of tachykinin receptors within baroreflex afferent pathway and neurocontrol of blood pressure regulation[Pubmed:29900692] |
| Journal of Veterinary Science | Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement[Pubmed: 30562932] |
| Respiratory Physiology & Neurobiology | Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma[Pubmed: 31542455] |
| Chinese Medicine | Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model[Pubmed: 33014123] |
| Medical Research Journal | Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy[] |
| Int Urol Nephrol | Evaluation of the causes affecting the development of pruritus in patients with peritoneal dialysis[34213714] |
| J Chem Neuroanat | Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice[34626771] |