线粒体超氧化物歧化酶(SOD2)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Superoxide Dismutase 2, Mitochondrial (SOD2)
IPO-B; MNSOD; Mn-SOD
- 编号KSB083Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.78-50ng/mL
- 可适灵敏度0.37ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6527¥ 10878¥ 19580¥ 38073¥ 65268
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对线粒体超氧化物歧化酶(SOD2)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB083Mu01 | 线粒体超氧化物歧化酶(SOD2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB083Mu01 | 线粒体超氧化物歧化酶(SOD2)活性蛋白 | Cell culture; Activity Assays. |
| PAB083Mu01 | 线粒体超氧化物歧化酶(SOD2)多克隆抗体 | WB |
| LAB083Mu81 | 线粒体超氧化物歧化酶(SOD2)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB083Mu71 | 线粒体超氧化物歧化酶(SOD2)多克隆抗体(生物素标记) | WB |
| SEB083Mu | 线粒体超氧化物歧化酶(SOD2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| KSB083Mu01 | 线粒体超氧化物歧化酶(SOD2)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
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| Anticancer Research | Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer[Pubmed:25550558] |
| Arch Oral Biol | Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes[PubMed: 26143097] |
| J Oral Pathol Med | Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva[PubMed: 26608886] |
| Free Radic Biol Med. | DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts.[Pubmed:26806551] |
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| Journal of Pharmacological Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome[Pubmed: 30342783] |
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| PHARMACOLOGICAL RESEARCH | A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 Signaling[Pubmed: 32135248] |
| Biomolecules | Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development[Pubmed: 32751983] |
| Nutrients | Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡[34064937] |
| Pharmaceuticals | Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients[34832849] |
| Biochimica et Biophysica Acta-Molecular Basis of Disease | α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet[Pubmed:35569738] |