表面活性物质关联蛋白B(SP-B)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Surfactant Protein B (SP-B)

SFTPB; PSPB; SFTB3; SFTP3; SPB; Surfactant Associated Protein B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein

特异性

试剂盒中的抗体对表面活性物质关联蛋白B(SPB)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Rattus norvegicus (Rat,大鼠)应用(仅供研究使用,不用于临床诊断!)
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参考文献

杂志参考文献
Respiratory Physiology & NeurobiologyKinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery[PubMed: 21736957]
American Journal of Rhinology & AllergyDetection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps[PubMed: 23406594]
19Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study[PubMed: 20650982]
Plos oneStaphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins[PubMed: PMC3551896]
Plos oneThe Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS[PubMed: PMC3787032]
Cancer Cell.Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models[PubMed: PMC3406925]
Respir Physiol NeurobiolAcute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project[Pubmed: 23619193]
PLOS ONESurfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure[Pubmed:25514679]
Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge[Opus4:Source]
Int J CardiolPlasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study[PubMed: 26310985]
Plos OneSerum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE)[Pubmed:27337142]
PLoS OneThe Cerebral Surfactant System and Its Alteration in HydrocephalicConditions.[pubmed:27656877]
Frontiers in Aging NeuroscienceCorrelations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid.[pubmed:28101052]
Frontiers in Aging NeuroscienceCorrelations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid[PMC5209370]
Respiratory Physiology & NeurobiologyDiving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers[pubmed:28467885]
Cellular Physiology and BiochemistrySurfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production[pubmed:28743125]
American Journal of Physiology. Lung Cellular and Molecular PhysiologyChronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency.[pubmed:29167126]
Respiratory researchEffects and molecular mechanisms of intrauterine infection/inflammation on lung development[Pubmed:29747649]
Respiratory Physiology & Neurobiology Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan[Pubmed:29548887]
Lung Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation[Pubmed:29445934]
American Journal of Physiology Lung Cellular andMolecular PhysiologyGeneration of an alveolar epithelial type II cell line from induced pluripotent stem cells[Pubmed: 30211653]
Respiratory ResearchCulture of human alveolar epithelial type II cells by sprouting[Pubmed: 30340591]