转化生长因子β3(TGFb3)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Transforming Growth Factor Beta 3 (TGFb3)
TGF-B3; LAP; Latency-associated peptide
- 编号KSB949Ra01
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围15.62-1000pg/mL
- 可适灵敏度6.3pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7798¥ 12996¥ 23393¥ 45486¥ 77976
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特异性
试剂盒中的抗体对转化生长因子β3(TGFb3)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APB949Ra01 | 转化生长因子β3(TGFb3)活性蛋白 | Cell culture; Activity Assays. |
| RPB949Ra01 | 转化生长因子β3(TGFb3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB949Ra01 | 转化生长因子β3(TGFb3)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB949Ra21 | 转化生长因子β3(TGFb3)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB949Ra | 转化生长因子β3(TGFb3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB949Ra | 转化生长因子β3(TGFb3)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB949Ra | 转化生长因子β3(TGFb3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB949Ra01 | 转化生长因子β3(TGFb3)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells[PubMed: 25799173] |
| Fertility and Sterility | Influence of vitamin D and transforming growth factor β3 serum concentrations, obesity, and family history on the risk for uterine fibroids[pubmed:27743697] |
| Eur J Pharm Biopharm | New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation[pubmed:28087378] |
| Transforme edici büyüme faktörü-beta-1 nötralizan antikoru ve transforme edici büyüme faktörü-beta-3'ün trakeal darlık gelişimi üzerine etkileri[10.5606/tgkdc.dergisi.2017.14302] | |
| Fertility and Sterility | Ulipristal acetate decreases transforming growth factor β3 serum and tumor tissue concentrations in patients with uterine fibroids[Pubmed:29525690] |
| Scientific Reports | TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration[Pubmed:29795291] |
| Immunology Letters | The involvement of multifunctional TGF-β and related cytokines in pathogenesis of endometriosis[Pubmed: 30367890] |
| Cell Reports | Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue[Pubmed: 30332637] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Temporal TGF-β Supergene Family Signalling Cues Modulating Tissue Morphogenesis: Chondrogenesis within a Muscle Tissue Model?[Pubmed: 32660137] |
| Equine Vet J | Limited added value of negative pressure wound therapy compared to calcium alginate dressings for second intention healing in a non©\contaminated and?¡[34115409] |
| AAPS PharmSciTech | Nanoparticles and Nanostructured Films with TGF-β3: Preparation, Characterization, and Efficacy[34378118] |