武汉云克隆科技股份有限公司

心肌肌钙蛋白T(TNNT2)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Troponin T Type 2, Cardiac (TNNT2)

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T

  • 心肌肌钙蛋白T(TNNT2)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 心肌肌钙蛋白T(TNNT2)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对心肌肌钙蛋白T(TNNT2)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种：Rattus norvegicus (Rat，大鼠)应用(仅供研究使用，不用于临床诊断！)
RPD232Ra01心肌肌钙蛋白T(TNNT2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAD232Ra01心肌肌钙蛋白T(TNNT2)多克隆抗体WB; IHC; ICC; IP.
MAD232Ra21心肌肌钙蛋白T(TNNT2)单克隆抗体WB; IHC; ICC; IP.
MED232Ra心肌肌钙蛋白T(TNNT2)检测试剂盒(酶联免疫吸附试验法,小样本)Enzyme-linked immunosorbent assay for Antigen Detection.
SED232Ra心肌肌钙蛋白T(TNNT2)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCD232Ra心肌肌钙蛋白T(TNNT2)检测试剂盒(化学发光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMD232Ra心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSD232Ra01心肌肌钙蛋白T(TNNT2)检测试剂盒DIY材料(酶联免疫吸附试验法)自己动手制作ELISA试剂盒的主要材料

参考文献

杂志参考文献
Basic & Clinical Pharmacology & ToxicologyCardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in Rats[PubMed: 21232020]
Basic & Clinical Pharmacology & ToxicologyCardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats[Wiley: source]
European Journal of PharmacologyProtective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats[ScienceDirect: S0014299909009984]
Journal of translational medicineThe altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury[PubMed: PMC3694448]
Oral Diseases Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction[Pubmed: 22834943]
An International Journal of Pharmaceutical SciencesIn vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium[Pubmed: 23700796]
Journal of Animal and Feed Sciences,The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats[Pl:Source]
Liver Int.Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis[Pubmed:24330504]
Cardiovasc Toxicol. Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs[Pubmed:24420420]
British Journal of PharmacologyGlutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise[Pubmed:25039894]
Chemico-Biological InteractionsCrocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways[Pubmed:26807765]
The Journal of Pharmacology and Experimental TherapeutiesThe Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice[Pubmed:27247000]
The Journal of Maternal-Fetal & Neonatal MedicineTroponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies.[pubmed:27937003]
PLoS One. Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice.[pubmed:27907155]
Biomedicine & PharmacotherapyRosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats[pubmed:28076830]
PLoS OneImatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model[pubmed:28570599]
EXPERIMENTAL AND THERAPEUTIC MEDICINE Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus[pubmed:29042932]
PLoS OneIncreases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury[Pubmed: 30192780]
PLoS OneTissue suction-mediated gene transfer to the beating heart in mice[Pubmed: 32027678]
Journal of Human KineticsHormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy[Pubmed: 32148580]
BIOMEDICINE & PHARMACOTHERAPYChlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress[Pubmed: 33022535]
ToxicsNorepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats[Pubmed: 32947820]
ElifeIron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice[33526170]
Cell MetabolismPharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species[34536344]
Anal Bioanal ChemDevelopment of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications[34673993]
Experimental and Therapeutic MedicineHydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest[Pubmed:35495584]