胰蛋白酶原激活肽(TAP)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP)
- 编号KSA634Hu11
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围123.5-10000pg/mL
- 可适灵敏度54.1pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6804¥ 11340¥ 20412¥ 39690¥ 68040
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对胰蛋白酶原激活肽(TAP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA634Hu01 | 胰蛋白酶原激活肽(TAP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA634Hu21 | 胰蛋白酶原激活肽(TAP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA634Hu11 | 胰蛋白酶原激活肽(TAP)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA634Hu01 | 胰蛋白酶原激活肽(TAP)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA634Hu71 | 胰蛋白酶原激活肽(TAP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA634Hu21 | 胰蛋白酶原激活肽(TAP)单克隆抗体 | WB; IHC; ICC; IP. |
| CEA634Hu | 胰蛋白酶原激活肽(TAP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA634Hu | 胰蛋白酶原激活肽(TAP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA634Hu11 | 胰蛋白酶原激活肽(TAP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Diabetologia | Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice[SpringerLink: x4l01435t34452jr] |
| Molecular Biology Reports | Prior peritoneal lavage with hot 0.9% saline induces HSP70 expression and protects against cerulein-induced acute pancreatitis in rats[Pubmed: 23096089] |
| Journal of the Pancreas | Case report of mannose-binding lectin (MBL) deficiency and postoperative sepsis and coagulopathy in a patient following total pancreatectomy for chronic pancreatitis.[Pubmed:25262717] |
| Journal of Gastroenterology | A small molecule inhibitor of NFκB blocks ER stress and the NLRP3 inflammasome and prevents progression of pancreatitis[pubmed:27418337] |
| Indian Journal of Pharmaceutical Sciences | Effects of Calcium Channel Blockers on Trypsinogen Activation and Severity of Cerulein-induced Acute Pancreatitis in Rats[IJPS:Source] |
| Tropical Journal of Pharmaceutical Research | Investigation of non-pharmaceutical cures for acute pancreatitis induced by cerulein in rats[] |
| Pancreas | The Regulatory Effect of the Kinase Inhibitor PD98059 on Autophagic Flux During Trypsinogen Activation in Pancreatic Acinar Cells[Pubmed: 32011537] |