武汉云克隆科技股份有限公司

类胰蛋白酶(TPS)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Tryptase (TPS)

TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III

  • 类胰蛋白酶(TPS)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 类胰蛋白酶(TPS)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 类胰蛋白酶(TPS)检测试剂盒DIY材料(酶联免疫吸附试验法)产品图片
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对类胰蛋白酶(TPS)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
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参考文献

杂志参考文献
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J Pediatr. Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2[Pubmed:24530123]
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PLOS ONEA Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats[Pubmed:25541997]
Neurogastroenterology & MotilityPeritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery[Pubmed:25677271]
Mediators InflammElevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report[PubMed: 26089601]
Int ImmunopharmacolTryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis[PubMed: 26049029]
Mediators of InflammationAltered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins[Journals: Mi]
Mediators of InflammationAltered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins[Pubmed:27057098]
Analytica Chimica ActaAn ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis[]
Journal of Clinical InvestigationDengue virus-elicited tryptase induces endothelial permeability and shock[Pubmed: 31265436]
Frontiers in PhysiologyMast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function[Pubmed: 31998139]
Biomarkers of Allergic Asthma and their Association with Serum Parameters[]
Annals of Tropical Medicine & Public HealthEffectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity[]
Front ImmunolSerum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive …[34650557]
Microbiol SpectrMast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome[Pubmed:35612327]