肿瘤坏死因子β(TNFb)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Tumor Necrosis Factor Beta (TNFb)
LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha
- 编号KSA134Ra01
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围7.8-500pg/mL
- 可适灵敏度3.3pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对肿瘤坏死因子β(TNFb)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA134Ra01 | 肿瘤坏死因子β(TNFb)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA134Ra02 | 肿瘤坏死因子β(TNFb)活性蛋白 | Cell culture; Activity Assays. |
| RPA134Ra02 | 肿瘤坏死因子β(TNFb)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA134Ra01 | 肿瘤坏死因子β(TNFb)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA134Ra02 | 肿瘤坏死因子β(TNFb)多克隆抗体 | WB |
| MAA134Ra21 | 肿瘤坏死因子β(TNFb)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA134Ra | 肿瘤坏死因子β(TNFb)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA134Ra | 肿瘤坏死因子β(TNFb)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA134Ra01 | 肿瘤坏死因子β(TNFb)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
| CGA134Ra02 | 肿瘤坏死因子β(TNFb)cDNA | PCR、蛋白表达等 |
参考文献
| 杂志 | 参考文献 |
| Journal of Applied Physiology | Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process[PubMed: 22984252] |
| Journal of Translational Medicine | Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation[Pubmed:26847569] |
| International Journal of Stem Cell Research and Transplantation | Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines[IJST-2328-3548-04-803] |
| Probiotics Antimicrob Proteins | Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …[Pubmed:29305686] |
| Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …[Pubmed: 32680553] |
| Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort[] | |
| BMC MUSCULOSKELETAL DISORDERS | Elevated lymphotoxin-¦Á (TNF¦Â) is associated with intervertebral disc degeneration[33441130] |