血管非炎性蛋白1(VNN1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Vanin 1 (VNN1)
Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1
- 编号KSC598Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, urine, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.059ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7560¥ 12600¥ 22680¥ 44100¥ 75600
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对血管非炎性蛋白1(VNN1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC598Hu02 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu03 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu01 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu05 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu04 | 血管非炎性蛋白1(VNN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC598Hu02 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC598Hu04 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC598Hu03 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB |
| PAC598Hu01 | 血管非炎性蛋白1(VNN1)多克隆抗体 | WB |
| LAC598Hu71 | 血管非炎性蛋白1(VNN1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAC598Hu24 | 血管非炎性蛋白1(VNN1)单克隆抗体 | WB |
| MAC598Hu21 | 血管非炎性蛋白1(VNN1)单克隆抗体 | WB |
| MAC598Hu22 | 血管非炎性蛋白1(VNN1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC598Hu | 血管非炎性蛋白1(VNN1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC598Hu | 血管非炎性蛋白1(VNN1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSC598Hu01 | 血管非炎性蛋白1(VNN1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Toxicology | Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury[ScienceDirect: S0300483X11003234] |
| Journal of Pharmacology and Experimental Therapeutics | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury[Aspet: 192807] |
| J Pharmacol Exp Ther | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury.[PubMed: 22399813] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis[PubMed: 23307618] |
| Iranian Journal of Health and Physical Activity | Effects of Exercise and Ferula Gummosa on Apelin of Cardiac and Kidney Tissues in L-NAME induced Hypertension in Rats[Jm.um: Source] |
| Platelets | Value of vanin-1 assessment in adult patients with primary immune thrombocytopenia[Pubmed: 23534352] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis.[Pubmed: 23307618] |
| Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats[Sagepub:Source] |
| Food Chem Toxicol. | The effect of activated charcoal on adenine-induced chronic renal failure in rats[Pubmed:24412558] |
| Hypertension Research | Early urinary biomarkers for renal tubular damage in spontaneously hypertensive rats on a high salt intake[PubMed: 26376947] |
| Inflammation | Citrate Attenuates Adenine-Induced Chronic Renal Failure in Rats by Modulating the Th17/Treg Cell Balance[PubMed: 26253296] |
| Toxicology | Early prediction of cisplatin-induced nephrotoxicity by urinary vanin-1 in patients with urothelial carcinoma[Pubmed:27317936] |
| J Biomed Mater Res A | Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice[Pubmed:26850371] |
| APMIS | Citrate attenuates vascular calcification in chronic renal failure rats.[pubmed:28332248] |
| Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.[pubmed:29027259] |
| Cellular Physiology and Biochemistry | Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats …[Pubmed:29550811] |
| International Journal of Molecular Sciences | Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis[Pubmed: 30332759] |
| International Journal of Molecular Sciences | Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive Rats[Pubmed: 31514290] |
| The Journal of Clinical Hypertension homepage | Urinary vanin‐1 associated with chronic kidney disease in hypertensive patients: A pilot study[Pubmed: 32869948] |
| J Clin Hypertens | Association of urinary vanin©\1 with kidney function decline in hypertensive patients[34029440] |
| Plasma vanin-1 as a novel biomarker of sepsis for trauma patients: a prospective multicenter cohort study[33624223] |