玻连蛋白(VTN)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Vitronectin (VTN)
V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
- 编号KSB041Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围6.25-400ng/mL
- 可适灵敏度2.74ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7182¥ 11970¥ 21546¥ 41895¥ 71820
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对玻连蛋白(VTN)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB041Hu02 | 玻连蛋白(VTN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB041Hu03 | 玻连蛋白(VTN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB041Hu01 | 玻连蛋白(VTN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB041Hu02 | 玻连蛋白(VTN)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB041Hu01 | 玻连蛋白(VTN)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB041Hu03 | 玻连蛋白(VTN)多克隆抗体 | WB; IHC; ICC; IP. |
| FAB041Hu01 | 抗玻连蛋白(VTN)多克隆抗体 | Flow cytometry. |
| FAB041Hu03 | 抗玻连蛋白(VTN)多克隆抗体 | Flow cytometry. |
| LAB041Hu71 | 玻连蛋白(VTN)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB041Hu22 | 玻连蛋白(VTN)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB041Hu | 玻连蛋白(VTN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB041Hu | 玻连蛋白(VTN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSB041Hu01 | 玻连蛋白(VTN)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB041Hu01 | 玻连蛋白(VTN)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer[IEEE: 6098563] |
| PLoS ONE | Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance[PubMed: PMC3754973] |
| Liver International | Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE[Pubmed: 23944848] |
| Analytical Biochemistry | A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots[Pubmed:29154788] |
| Journal of Materials Chemistry B | Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs[Doi: 10.1039/C8TB02283F ] |
| JOURNAL OF NEPHROLOGY | Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts[33275196] |