无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
- 编号KSP549Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.059ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7560¥ 12600¥ 22680¥ 44100¥ 75600
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对无翅型MMTV整合位点家族成员5A(WNT5A)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPP549Hu01 | 无翅型MMTV整合位点家族成员5A(WNT5A)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAP549Hu01 | 无翅型MMTV整合位点家族成员5A(WNT5A)多克隆抗体 | WB; IHC; ICC; IP. |
| MAP549Hu21 | 无翅型MMTV整合位点家族成员5A(WNT5A)单克隆抗体 | WB; IHC; ICC; IP. |
| SEP549Hu | 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMP549Hu | 无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSP549Hu01 | 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Liver International | Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis[Pubmed:25488180] |
| Journal of Physiology and Biochemistry | Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells[PubMed: 26547443] |
| Liver | Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis[PubMed: 25488180] |
| Transl Res | Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures[PubMed: 26163991] |
| 不确定杂志名 | Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an …[servlets:MCRFileNodeServlet] |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.[pubmed:28754618] |
| Arteriosclerosis, Thrombosis, and Vascular Biology | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With …[Pubmed:29674475] |
| Stem Cells | Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells[Pubmed:29377497] |
| Science Translational Medicine | Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidases[Pubmed: 31534019] |
| J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans[Pubmed: 30137542] |
| Cancer Research | Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis[Pubmed: 31932454] |
| CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease[Pubmed: 33188773] |
| Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and …[Pubmed: 32491086] |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties[34823885] |
| PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study[Pubmed:34990470] |
| J Clin Periodontol | Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study[Pubmed:35569034] |