抗心肌肌钙蛋白I抗体(Anti-cTnI)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Anti-Cardiac Troponin I Antibody (Anti-cTnI)
TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac
产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测抗心肌肌钙蛋白I抗体(Anti-cTnI)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的抗心肌肌钙蛋白I抗体(Anti-cTnI)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 87-101 | 92 |
| EDTA plasma(n=5) | 94-102 | 97 |
| heparin plasma(n=5) | 78-105 | 85 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的抗心肌肌钙蛋白I抗体(Anti-cTnI)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中抗心肌肌钙蛋白I抗体(Anti-cTnI)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-101% | 82-92% | 97-105% | 86-103% |
| EDTA plasma(n=5) | 84-104% | 80-104% | 93-101% | 94-102% |
| heparin plasma(n=5) | 81-93% | 93-101% | 97-105% | 79-89% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板5次；
5. 加TMB底物90µL，37°C孵育10-20分钟；
6. 加终止液50µL，立即450nm读数。
实验原理
将抗原包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的抗心肌肌钙蛋白I抗体(Anti-cTnI)与连接于固相载体上的抗原结合，然后加入HRP标记的二抗，彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗心肌肌钙蛋白I抗体(Anti-cTnI)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA478Hu02 | 心肌肌钙蛋白I(cTnI)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA478Hu61 | 心肌肌钙蛋白I(cTnI)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA478Hu01 | 心肌肌钙蛋白I(cTnI)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA478Hu01 | 心肌肌钙蛋白I(cTnI)活性蛋白 | Cell culture; Activity Assays. |
| APA478Hu61 | 心肌肌钙蛋白I(cTnI)活性蛋白 | Cell culture; Activity Assays. |
| PAA478Hu01 | 心肌肌钙蛋白I(cTnI)多克隆抗体 | WB; IHC |
| LAA478Hu71 | 心肌肌钙蛋白I(cTnI)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA478Hu28 | 心肌肌钙蛋白I(cTnI)单克隆抗体 | WB; IHC; ICC; IP. |
| RAA478Hu21 | 心肌肌钙蛋白I(cTnI)重组抗体 | WB; IHC; ICC. |
| MAA478Hu21 | 心肌肌钙蛋白I(cTnI)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA478Hu27 | 心肌肌钙蛋白I(cTnI)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA478Hu23 | 心肌肌钙蛋白I(cTnI)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA478Hu24 | 心肌肌钙蛋白I(cTnI)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA478Hu25 | 心肌肌钙蛋白I(cTnI)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA478Hu29 | 心肌肌钙蛋白I(cTnI)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA478Hu22 | 心肌肌钙蛋白I(cTnI)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA478Hu26 | 心肌肌钙蛋白I(cTnI)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA478Hu08 | 抗心肌肌钙蛋白I(cTnI)单克隆抗体 | FCM |
| FAA478Hu06 | 抗心肌肌钙蛋白I(cTnI)单克隆抗体 | Flow cytometry. |
| FAA478Hu04 | 抗心肌肌钙蛋白I(cTnI)单克隆抗体 | Flow cytometry. |
| FAA478Hu02 | 抗心肌肌钙蛋白I(cTnI)单克隆抗体 | Flow cytometry. |
| LAA478Hu74 | 心肌肌钙蛋白I(cTnI)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA478Hu | 心肌肌钙蛋白I(cTnI)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA478Hu | 心肌肌钙蛋白I(cTnI)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA478Hu | 抗心肌肌钙蛋白I抗体(Anti-cTnI)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA478Hu | 心肌肌钙蛋白I(cTnI)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA478Hu | 心肌肌钙蛋白I(cTnI)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA478Hu01 | 心肌肌钙蛋白I(cTnI)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Animal and Feed Sciences, | The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats[Pl:Source] |
| Interact Cardiovasc Thorac Surg. | Comparison of femoral and aortic remote ischaemia preconditioning for cardioprotection against myocardial ischaemia/reperfusion injury in a rat model[Pubmed:25205781] |
| Exp Ther Med | Cardioprotective effect of remote preconditioning of trauma and remote ischemia preconditioning in a rat model of myocardial ischemia/reperfusion injury[PubMed: 26136887] |
| Heart International | Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fraction[pubmed:27924211] |
| Archivos de medicina veterinaria | Cardiac biomarkers in dogs with visceral leishmaniasis[S0301-732X2016000300004] |
| PLOS Pathogens | Cathepsin B aggravates coxsackievirus B3-induced myocarditis through activating the inflammasome and promoting pyroptosis[Pubmed:29360865] |
| Science of the Total Environment | Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and[Pubmed:29727991] |
| Molecules | Melatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy[Pubmed:29547569] |
| Molecules | The Multivariate Regression Statistics Strategy to Investigate Content-Effect Correlation of Multiple Components in Traditional Chinese Medicine Based on a …[Pubmed:29494523] |
| Molecular Medicine Reports | Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion[Pubmed:29393473] |
| Molecular Medicine Reports | Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats[Pubmed:29956781] |
| Biological & Pharmaceutical Bulletin | Protective Effects of Shenfu Injection against Myocardial Ischemia-Reperfusion Injury via Activation of eNOS in Rats[Pubmed:29910216] |
| Oncology Letters | Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of …[Pubmed:29928398] |
| BMC Pharmacology and Toxicology | Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis[Pubmed:29321061] |
| FASEB Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure[Pubmed: 30230922] |
| Frontiers in Pharmacology | Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway[Pubmed: 30890937] |
| canadian journal of physiology and pharmacology | Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats?[Pubmed: 30840489] |
| Journal of Cellular Physiology | Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes[Pubmed: 30982981] |
| BIOSENS BIOELECTRON | Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA …[Pubmed: 30954926] |
| Life Sciences | Protective effects of two novel nitronyl nitroxide radicals on heart failure induced by hypobaric hypoxia[Pubmed: 31102744] |
| Mikrochim Acta | DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label[Pubmed: 31123904] |
| Biosensors and Bioelectronics | DNA nanotetrahedron-assisted electrochemical aptasensor for cardiac troponin I detection based on the co-catalysis of hybrid nanozyme, natural enzyme and artificial …[] |
| Biofactors | MiR‐183‐5p protects rat hearts against myocardial ischemia/reperfusion injury through targeting VDAC1[Pubmed: 31618500] |
| Nature Communications | Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb[Pubmed: 32161256] |
| BIOMEDICINE & PHARMACOTHERAPY | Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress[Pubmed: 33022535] |
| Pharmacol Res | Therapeutic potential of cPLA2 inhibitor to counteract dilated-cardiomyopathy in cholesterol-treated H9C2 cardiomyocyte and MUNO rat[Pubmed: 32942017] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Early Elevation of Systemic Plasma Clusterin after Reperfused Acute Myocardial Infarction in a Preclinical Porcine Model of Ischemic Heart Disease[Pubmed: 32605184] |
| Eur J Pharmacol | NLRX1 knockout aggravates lipopolysaccharide (LPS)-induced heart injury and attenuates the anti-LPS cardioprotective effect of CYP2J2/11, 12-EET by enhancing …[Pubmed: 32574674] |
| American Journal of Translational Research | Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and …[Pubmed: 32509190] |
| Arabian Journal for Science and Engineering | Ethanolic and Aqueous Extracts of Avocado (Persea americana) Seeds Attenuates Doxorubicin-Induced Cardiotoxicity in Male Albino Rats[] |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes[] | |
| Hum Exp Toxicol | Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats[33882726] |
| Curr Protoc | Isolation and Culture of Neonatal Murine Primary Cardiomyocytes[34289259] |
| Cell Metabolism | Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species[34536344] |
| Biomed Pharmacother | Pea (Pisum sativum) peel extract attenuates DOX-induced oxidative myocardial injury[34649330] |
| Anal Bioanal Chem | Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications[34673993] |
| Biomed Res Int | Nicotinic Acid Riboside Regulates Nrf-2/P62-Related Oxidative Stress and Autophagy to Attenuate Doxorubicin-Induced Cardiomyocyte Injury[Pubmed:35242876] |
| Talanta | A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein[Pubmed:35714415] |
| Nutrients | Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733[Pubmed:35215383] |
