不对称二甲基精氨酸(ADMA)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Asymmetrical Dimethylarginine (ADMA)
N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine
- 编号CEB301Ge
- 物种Pan-species (General，通用)相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围12.35-1,000ng/mL
- 灵敏度最小可检测剂量小于等于4.99ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 3392¥ 4845¥ 21803¥ 41183¥ 339150
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产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测不对称二甲基精氨酸(ADMA)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的不对称二甲基精氨酸(ADMA)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 78-92 | 87 |
| EDTA plasma(n=5) | 92-101 | 96 |
| heparin plasma(n=5) | 96-103 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的不对称二甲基精氨酸(ADMA)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中不对称二甲基精氨酸(ADMA)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-101% | 85-97% | 98-105% | 93-101% |
| EDTA plasma(n=5) | 86-96% | 84-101% | 91-98% | 95-102% |
| heparin plasma(n=5) | 85-102% | 98-105% | 83-92% | 90-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将不对称二甲基精氨酸(ADMA)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPB301Ge11 | 不对称二甲基精氨酸(ADMA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPB301Ge21 | 不对称二甲基精氨酸(ADMA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAB301Ge01 | 不对称二甲基精氨酸(ADMA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAB301Ge71 | 不对称二甲基精氨酸(ADMA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| CEB301Ge | 不对称二甲基精氨酸(ADMA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB301Ge | 不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB301Ge11 | 不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| PLoS One. | Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in Highlanders[PubMed: PMC3439466] |
| Cytokine | Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats.[Pubmed: 23834755] |
| Neurol Res. | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats.[Pubmed:25475507] |
| Int J Clin Exp Med. | Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients[Pubmed:25550968] |
| Clin Respir J | Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD[PubMed: 26076870] |
| Cytokine | Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive rats[PubMed: 26375520] |
| Neurological Research | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats[PubMed: 25475507] |
| Scientific Reports | Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study[pubmed:27677852] |
| clinica chimica acta | Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease.[pubmed:27884754] |
| The Journal of Physiology | Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans.[pubmed:26840628] |
| Angiogenesis | Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA).[pubmed:29150732] |
| Scientific Reports | A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling[Pubmed:29523851] |
| Mechanisms of Ageing and Development | Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territories[Pubmed:29605639] |
| Nutrition, Metabolism and Cardiovascular Diseases | Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns[Doi: 10.1016/j.numecd.2018.09.002] |
| Biochemical and Biophysical Research Communications | Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats[Pubmed: 30454888] |
| Brazilian Journal of Pharmaceutical Science | Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients[] |
| ACS Combinatorial Science | Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathway[Pubmed: 30673277] |
| International Journal of the Cardiovascular Academy | Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats[] |
| Oxid Med Cell Longev | The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT Cells[Pubmed: 32104540] |
| Molecular Medicine Reports | Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in …[Pubmed: 31746378] |
| International Journal of Nephrology | Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis Patients[Pubmed: 33163233] |
| Journal of Clinical Medicine | The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial[Pubmed: 32344735] |
| Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub | Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls[33463628] |
| WIENER KLINISCHE WOCHENSCHRIFT | Non-functioning adrenal incidentalomas may increase toxic metabolites[34255169] |
| pharmaceuticals | Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats—A Comparison with Mesna[34959638] |
| Int J Biochem Cell Biol | HIF-1α and Nrf2 regulates hypoxia induced overexpression of DDAH1 through promoter activation in prostate cancer[Pubmed:35644470] |
