表面活性物质关联蛋白A(SFTPA1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Pulmonary Surfactant Associated Protein A1 (SFTPA1)
PRL; SFTPA; PSAP; PSPA; SFTP; SPA; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein; Surfactant Associated Protein A
- 编号SEA890Ca
- 物种Canis familiaris; Canine (Dog，犬)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围6.25-400pg/mL
- 灵敏度最小可检测剂量小于等于2.73pg/mL.
- 样本类型serum, plasma, lung lavage fluid, tissue homogenates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 3024¥ 4320¥ 19440¥ 36720¥ 302400
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特异性
本试剂盒用于检测表面活性物质关联蛋白A(SFTPA1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的表面活性物质关联蛋白A(SFTPA1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 79-91 | 84 |
| EDTA plasma(n=5) | 94-102 | 98 |
| heparin plasma(n=5) | 98-105 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的表面活性物质关联蛋白A(SFTPA1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中表面活性物质关联蛋白A(SFTPA1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-101% | 78-95% | 88-102% | 78-90% |
| EDTA plasma(n=5) | 94-103% | 86-101% | 88-97% | 98-105% |
| heparin plasma(n=5) | 87-101% | 97-104% | 80-103% | 78-90% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将表面活性物质关联蛋白A(SFTPA1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的表面活性物质关联蛋白A(SFTPA1)与连接于固相载体上的抗体结合，然后加入生物素化的表面活性物质关联蛋白A(SFTPA1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的表面活性物质关联蛋白A(SFTPA1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Canis familiaris; Canine (Dog，犬) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA890Ca01 | 表面活性物质关联蛋白A(SFTPA1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA890Ca01 | 表面活性物质关联蛋白A(SFTPA1)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA890Ca21 | 表面活性物质关联蛋白A(SFTPA1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA890Ca | 表面活性物质关联蛋白A(SFTPA1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA890Ca | 表面活性物质关联蛋白A(SFTPA1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| The American Journal of Rhinology & Allergy | Surfactant protein A and D in chronic rhinosinusitis with nasal polyposis and corticosteroid response[Ingenta: art00005] |
| American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps[PubMed: 23406594] |
| Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins[PubMed: PMC3551896] |
| Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS[PubMed: PMC3787032] |
| Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge[Opus4:Source] | |
| Mol Med Rep | Expression of surfactant protein-A in exhaled breath condensate of patients with chronic obstructive pulmonary disease[PubMed: 26707652] |
| Plos One | Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE)[Pubmed:27337142] |
| PLoS One | The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions.[pubmed:27656877] |
| Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid.[pubmed:28101052] |
| Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid[PMC5209370] |
| American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency.[pubmed:29167126] |
| Molecular biosystems | Metabolic profiling of asthma in mice and the interventional effects of SPA using liquid chromatography and Q-TOF mass spectrometry[pubmed:28463380] |
| PLoS One | Evaluation of surfactant proteins A, B, C, and D in articular cartilage, synovial membrane and synovial fluid of healthy as well as patients with osteoarthritis and …[Pubmed: 30235245] |
| PLOS ONE | Organic barn dust inhibits surfactant protein D production through protein kinase-c alpha dependent increase of GPR116[Pubmed: 30543664] |
| Molecular Neurobiology | Elevated levels of multifunctional surfactant proteins in cerebrospinal fluid are associated with signs of increased cerebrospinal fluid flow in cranial magnetic …[Doi: 10.1007/s12035-017-0835-5] |
| Molecular Neurobiology | Rheologically Essential Surfactant Proteins of the CSF Interacting with Periventricular White Matter Changes in Hydrocephalus Patients–Implications for CSF …[Doi: 10.1007/s12035-019-01648-z] |
| Antioxidants (Basel) | Oxidative Stress and Inflammatory Mediators in Exhaled Breath Condensate of Patients with Pulmonary Tuberculosis. A Pilot Study with a Biomarker Perspective[34679707] |
