武汉云克隆科技股份有限公司

18kDa阳离子抗菌肽(CAMP)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP)

CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic

  • 18kDa阳离子抗菌肽(CAMP)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 18kDa阳离子抗菌肽(CAMP)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 18kDa阳离子抗菌肽(CAMP)检测试剂盒(酶联免疫吸附试验法)实验结果图
  • SEC419Ra.jpg标准曲线图
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测18kDa阳离子抗菌肽(CAMP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的18kDa阳离子抗菌肽(CAMP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)96-105101
EDTA plasma(n=5)92-10397
heparin plasma(n=5)95-105101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的18kDa阳离子抗菌肽(CAMP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中18kDa阳离子抗菌肽(CAMP)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)92-101%92-101%88-97%81-94%
EDTA plasma(n=5)81-96%99-105%85-96%95-104%
heparin plasma(n=5)83-90%85-99%92-99%78-99%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将18kDa阳离子抗菌肽(CAMP)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的18kDa阳离子抗菌肽(CAMP)与连接于固相载体上的抗体结合，然后加入生物素化的18kDa阳离子抗菌肽(CAMP)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的18kDa阳离子抗菌肽(CAMP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号适用物种：Rattus norvegicus (Rat，大鼠)应用(仅供研究使用，不用于临床诊断！)
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参考文献

杂志参考文献
University of Waikato The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis[Waikato:Source]
Clin Infect Dis.Host immune response to tuberculous meningitis[Pubmed:25301213]
VaccineOn birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment[Pubmed:25444792]
UrologyModulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells [Pubmed:25681250]
UrologyModulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells[PubMed: 25681250]
Sarcoidosis Vasc Diffuse Lung DisCathelicidin as a link between sarcoidosis and tuberculosis[PubMed: 26422567]
Allergy and Asthma ProceedingsThe association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children[PubMed: 26108071]
J Clin Microbiol.Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis.[Pubmed:27008883]
experimental dermatologyLight-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin.[pubmed:27315464]
PLOS ONEReduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension.[pubmed:27391973]
Front Pharmacol. Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance.[pubmed:28261091]
Allergologia et immunopathologiaThe relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin[pubmed:27955890]
Chinese Journal of Osteoporosis and Bone Mineral ResearchEffects of cold exposure on the bone marrow adipose tissue[DOI: 10.3969/j.issn.1674-2591.2017.05.008]
Scientific ReportsMeasurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique[Pubmed:29374283]
Experimental ParasitologyAntimalarial activity of vitamin D3 (VD3) does not result from VD3-induced antimicrobial agents including nitric oxide or cathelicidin[Pubmed: 30904694]
TheranosticsMyeloid cell-derived LL-37 promotes lung cancer growth by activating Wnt/β-catenin signaling[Pubmed: 31149039]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCESShort-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37[Pubmed: 32050475]
MICROB PATHOGENESISHypovitaminosis D and reduced cathelicidin are strongly correlated during the multidrug therapy against leprosy[Pubmed: 32645421]
International Journal of NanomedicineEffect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide[Pubmed: 32606669]
博士论文Relações causais entre a expressão dos genes do receptor de Vitamina D e do Peptídeo Antimicrobiano Catelicidina sobre marcadores sorológicos de pessoas com …[]
PLoS OneBeneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies[33999928]
New MedAnalysis of the level of non-specific and specific immunity parameters in saliva of children with osteogenesis imperfecta and study of relationships between?¡­[]
Type I interferons link skin-associated dysbiotic commensal bacteria to pathogenic inflammation and angiogenesis in rosacea[]
Int J Mol SciCompartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual Infection[Pubmed:35328414]
Cell RepLL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity[Pubmed:35649354]