细胞色素C氧化酶亚基Ⅱ(COX2)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Cytochrome C Oxidase Subunit II (COX2)
MTCO2; MT-CO2; COII; COXII; Mitochondrially Encoded Cytochrome C Oxidase II; Cytochrome c oxidase polypeptide II
产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测细胞色素C氧化酶亚基Ⅱ(COX2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将细胞色素C氧化酶亚基Ⅱ(COX2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的细胞色素C氧化酶亚基Ⅱ(COX2)与连接于固相载体上的抗体结合，然后加入生物素化的细胞色素C氧化酶亚基Ⅱ(COX2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的细胞色素C氧化酶亚基Ⅱ(COX2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD284Hu01 | 细胞色素C氧化酶亚基Ⅱ(COX2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| SED284Hu | 细胞色素C氧化酶亚基Ⅱ(COX2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD284Hu | 细胞色素C氧化酶亚基Ⅱ(COX2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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