内皮细胞特异分子1(ESM1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1)
Endocan
- 编号SEC463Hu
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.113ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 2940¥ 4200¥ 18900¥ 35700¥ 294000
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特异性
本试剂盒用于检测内皮细胞特异分子1(ESM1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的内皮细胞特异分子1(ESM1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 79-103 | 90 |
| EDTA plasma(n=5) | 93-101 | 97 |
| heparin plasma(n=5) | 81-95 | 85 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的内皮细胞特异分子1(ESM1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中内皮细胞特异分子1(ESM1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 83-96% | 82-93% | 98-105% |
| EDTA plasma(n=5) | 84-91% | 91-105% | 78-94% | 84-98% |
| heparin plasma(n=5) | 97-105% | 84-104% | 83-98% | 86-93% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将内皮细胞特异分子1(ESM1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的内皮细胞特异分子1(ESM1)与连接于固相载体上的抗体结合，然后加入生物素化的内皮细胞特异分子1(ESM1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的内皮细胞特异分子1(ESM1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APC463Hu01 | 内皮细胞特异分子1(ESM1)活性蛋白 | Cell culture; Activity Assays. |
| RPC463Hu01 | 内皮细胞特异分子1(ESM1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC463Hu01 | 内皮细胞特异分子1(ESM1)多克隆抗体 | WB |
| LAC463Hu71 | 内皮细胞特异分子1(ESM1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAC463Hu22 | 内皮细胞特异分子1(ESM1)单克隆抗体 | WB |
| MAC463Hu25 | 内皮细胞特异分子1(ESM1)单克隆抗体 | WB |
| SEC463Hu | 内皮细胞特异分子1(ESM1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC463Hu | 内皮细胞特异分子1(ESM1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Urology | Detection on dynamic changes of endothelial cell specific molecule-1 in acute rejection after renal transplantation[PubMed: 22608803] |
| Microvasc Res. | Endocan is useful biomarker of survival and severity in sepsis.[Pubmed: 24769132] |
| J Matern Fetal Neonatal Med. | Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromes[Pubmed:25211383] |
| Microvasc Res. | Endocan is useful biomarker of survival and severity in sepsis[Pubmed:24769132] |
| Angiology | Endocan Levels and Subclinical Atherosclerosis in Patients With Systemic Lupus Erythematosus[PubMed: 26614790] |
| Archives of Medical Science - Atherosclerotic Diseases | Assessment of subclinical cardiac damage in chronic plaque psoriasis patients: a case control study[Journal:-102] |
| Int Immunopharmacol. | Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension.[pubmed:27912147] |
| Oncotarget | Diagnostic and prognostic values of endothelial-cell-specific molecule-1 with malignant pleural effusions in patients with non-small cell lung cancer[pubmed:28514746] |
| Biomedical and Pharmacology Journal | Changes in Endocan Levels and Blood Coagulation in HIV Infection[DOI: 10.13005/bpj/1082] |
| Life Sciences | A novel role of endocan in alleviating LPS-induced acute lung injury[Pubmed:29627442] |
| International Urology and Nephrology | Is the plasma endocan level a reliable predictor for the severity of erectile dysfunction?[Pubmed:30054863] |
| The Open Rheumatology Journal | Serum Level of Endothelial Cell-Specific Molecule-1 (ESM-1) as a New Potential Biomarker for Rheumatoid Arthritis Disease Activity[Doi: 10.2174/1874312901812010189] |
| Molecular Medicine Reports | Downregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor‑κB activity[Pubmed: 30816462] |
| Biomarkers in Medicine | Endothelial biomarkers in the light of new sepsis definition[Pubmed: 30920847] |
| Experimental and Therapeutic Medicine | Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with …[] |
| Turkish Journal of Medical Sciences | A novel marker relationship between carotid intima–media thickness and disease activity score-28 in patients with rheumatoid arthritis: human endothelial cell …[Pubmed: 31655533] |
| Biomarkers | Role of Ascitic Endocan Levels in the Diagnosis of Spontaneous Bacterial Peritonitis in Decompensated Cirrhosis[Pubmed: 32364003] |
| J Obstet Gynaecol Res | Comparison of the maternal serum endocan levels in preterm premature rupture of membrane and normal pregnancy[34109715] |
| turkish journal clinics laboratory | The relationship between serum endocan levels with the presence of contrast-induced nephropathy in patients undergoing coronary angiography[] |
