武汉云克隆科技股份有限公司

磷脂酰肌醇蛋白聚糖3(GPC3)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Glypican 3 (GPC3)

DGSX; OCI5; SDYS; SGB; SGBS1; MXR7; Glypican Proteoglycan 3; GTR2-2; Intestinal protein OCI-5; Secreted glypican-3

  • 磷脂酰肌醇蛋白聚糖3(GPC3)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 磷脂酰肌醇蛋白聚糖3(GPC3)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 磷脂酰肌醇蛋白聚糖3(GPC3)检测试剂盒(酶联免疫吸附试验法)实验结果图
  • SEA971Ra.jpg标准曲线图
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测磷脂酰肌醇蛋白聚糖3(GPC3)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的磷脂酰肌醇蛋白聚糖3(GPC3)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)81-9793
EDTA plasma(n=5)86-10296
heparin plasma(n=5)99-105102

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的磷脂酰肌醇蛋白聚糖3(GPC3)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中磷脂酰肌醇蛋白聚糖3(GPC3)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)97-104%90-101%96-105%93-105%
EDTA plasma(n=5)85-94%85-101%79-92%96-103%
heparin plasma(n=5)86-96%80-93%89-104%85-93%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将磷脂酰肌醇蛋白聚糖3(GPC3)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的磷脂酰肌醇蛋白聚糖3(GPC3)与连接于固相载体上的抗体结合，然后加入生物素化的磷脂酰肌醇蛋白聚糖3(GPC3)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的磷脂酰肌醇蛋白聚糖3(GPC3)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号适用物种：Rattus norvegicus (Rat，大鼠)应用(仅供研究使用，不用于临床诊断！)
RPA971Ra01磷脂酰肌醇蛋白聚糖3(GPC3)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA971Ra01磷脂酰肌醇蛋白聚糖3(GPC3)多克隆抗体WB; IHC; ICC; IP.
MAA971Ra21磷脂酰肌醇蛋白聚糖3(GPC3)单克隆抗体WB; IHC; ICC; IP.
SEA971Ra磷脂酰肌醇蛋白聚糖3(GPC3)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA971Ra磷脂酰肌醇蛋白聚糖3(GPC3)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
Nature and ScienceDiagnostic Role of Serum Glypican-3 as a Tumor Marker for Hepatocellular Carcinoma [Sciencepub:10043238]
Journal of Applied Pharmaceutical ScienceCytotoxic effects of antiglypican-3 against HepG2 cell line[Ebscohost: Source]
Asian Pacific Journal of Cancer PreventionCan Glypican3 be Diagnostic for Early Hepatocellular Carcinoma among Egyptian Patients?[Pubmed: 24460300]
Clin Biochem.Diagnostic accuracy of serum glypican-3 for hepatocellular carcinoma: A systematic review and meta-analysis[Pubmed: 24362268]
Science and NatureDiagnostic Role of Serum Glypican-3 as Hepatocellular Carcinoma[Zolcat: Source]
Alexandria Journal of MedicineRole of serum glypican-3 in the diagnosis and differentiation of small hepatocellular carcinoma from hepatitis-C virus cirrhosis[ScienceDirect: S2090506814000062]
J Egypt Natl Canc Inst.Comparing prothrombin induced by vitamin K absence-II (PIVKA-II) with the oncofetal proteins glypican-3, Alpha feto protein and carcinoembryonic antigen in diagnosing hepatocellular carcinoma among Egyptian patients.[Pubmed:24841158]
European Journal of PharmacologyEvaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma[Pubmed:25449037]
Hepatobiliary Pancreat Dis IntPredictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern[abstract:abstract4525.shtml]
Clinical and Molecular Hepatology Glypican-3 level assessed by the enzyme-linked immunosorbent assay is inferior to alpha-fetoprotein level for hepatocellular carcinoma diagnosis[pmc:articles]
WileyGlypican‐3: A promising biomarker for hepatocellular carcinoma diagnosis and treatment[pubmed:28621802]
Biomedicine & PharmacotherapyCytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and[Pubmed:29669302]
Journal of Cancer and Clinical OncologyDevelopment and Validation of a Two-Site Immunoradiometric assay for Glypican-3 in plasma: Implication in Diagnosis of Hepatocellular Carcinoma[Researchgate:Source]
Turkish journal of oncologySerum Glypican-3, Vascular Endothelial Growth Factor, and Interleukin-6 Levels in Hepatocellular Carcinoma[10.5505:tjo.2018.1742]
Intensive Care Medicine ExperimentalElevated plasma glypicans are associated with organ failure in patients with infection[Pubmed: 30618011]
VALUE OF SERUM GLYPICAN 3 AS A PREDICTIVE TOOL FOR EARLY DIAGNOSIS OF HEPATOCELLULAR CARCINOMA (HCC)[]
ADVANCED RESEARCHESSearch for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C[]
Life SciSelenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and …[Pubmed:35640776]