铁调素(Hepc)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Hepcidin (Hepc)
HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor
- 编号SEB979Ra
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.064ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 3595¥ 5136¥ 23112¥ 43656¥ 359520
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测铁调素(Hepc)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的铁调素(Hepc)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-89 | 83 |
| EDTA plasma(n=5) | 80-90 | 85 |
| heparin plasma(n=5) | 97-105 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的铁调素(Hepc)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中铁调素(Hepc)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-102% | 90-103% | 94-104% | 79-102% |
| EDTA plasma(n=5) | 91-98% | 95-105% | 92-103% | 79-97% |
| heparin plasma(n=5) | 87-101% | 91-105% | 79-101% | 79-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将铁调素(Hepc)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的铁调素(Hepc)与连接于固相载体上的抗体结合，然后加入生物素化的铁调素(Hepc)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的铁调素(Hepc)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB979Ra01 | 铁调素(Hepc)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB979Ra01 | 铁调素(Hepc)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB979Ra21 | 铁调素(Hepc)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB979Ra | 铁调素(Hepc)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEB979Ra | 铁调素(Hepc)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB979Ra | 铁调素(Hepc)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB979Ra | 铁调素(Hepc)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB979Ra11 | 铁调素(Hepc)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| World J Gastroenterol. | Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis in rats[Pubmed: 24764672] |
| International Journal of Advanced Research | Modulations of serum hepcidin, IL6 and iron status by different exercise regimens in ovarectomized rats[Com:Source] |
| Neurochemical Journal | Serum iron metabolism markers including hepcidin in multiple sclerosis patients[Springer:Source] |
| Afro-Egypt J Infect Endem Dis | Monitoring Hepcidin Level in Chronic Hepatitis C Virus Patients during Therapy[Zu:Source] |
| Gastroenterology | Gluconeogenic signals regulate iron homeostasis via hepcidin in mice[Pubmed:Pmc3989026] |
| J Card Fail. | Inappropriate expression of hepcidin by liver congestion contributes to anemia and relative iron deficiency[Pubmed:24440572] |
| Food Funct. | The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemia[Pubmed:24752529] |
| Free Radic Res. | Changes in skeletal muscle iron metabolism outpace amyotrophic lateral sclerosis onset in transgenic rats bearing the G93A hmSOD1 gene mutation[Pubmed:25175826] |
| Saudi J Kidney Dis Transpl. | Hepcidin and regulation of iron homeostasis in maintenance hemodialysis patients[Pubmed:25193892] |
| J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders[Pubmed:25245337] |
| Gastroenterology Research and Practice | An Evaluation of the Correlation between Hepcidin Serum Levels and Disease Activity in Inflammatory Bowel Disease[Pubmed:25628652] |
| Clin Lab. | Serum hepcidin levels in Bulgarian population.[Pubmed:25651734] |
| The Tohoku Journal of Experimental Medicine | Liver Congestion in Heart Failure Contributes to Inappropriately Increased Serum Hepcidin despite Anemia[Pubmed:25742771] |
| Cancer Res | Hepcidin regulation in prostate and its disruption in prostate cancer[PubMed: 25858146] |
| Biochem Biophys Res Commun | B-cell translocation gene 2 promotes hepatic hepcidin production via induction of Yin Yang 1[PubMed: 25839654] |
| Blood Purif | Effect of a More Permeable Dialysis Membrane on ESA Resistance in Hemodialysis Patients - A Pilot Investigation[PubMed: 26536083] |
| Asian Journal of Clinical Nutrition | Urinary Hepcidin in Diagnosis of Iron Deficiency Anemia[Openview: E01Ad7C4A9109Ed5Fadb03Ec206D7Bdc] |
| Front Psychol | Ferrous sulfate, but not iron polymaltose complex, aggravates local and systemic inflammation and oxidative stress in dextran sodium sulfate-induced colitis in rats[PubMed: 25852597] |
| Biol Trace Elem Res | Iron Supplementation Attenuates the Inflammatory Status of Anemic Piglets by Regulating Hepcidin[PubMed: 25774043] |
| APS Journals | Kupffer cell depletion protects against the steatosis, but not the liver damage, induced by marginal copper, high fructose diet in male rats.[PubMed: 25813056] |
| European Journal of Haematology | Hepcidin in tumor‐related iron deficiency anemia and tumor‐related anemia of chronic disease: pathogenic mechanisms and diagnosis[PubMed: 24954786] |
| WJA | The Effects of Treatment on Serum Hepcidin and Iron Homeostasis in HIV-1-Infected In-dividuals[Journal: 59107] |
| Nutrition, Metabolism and Cardiovascular Diseases | Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case-control study.[Pubmed:27139516] |
| Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice[Pubmed:26757699] |
| Antioxid Redox Signal. | Hydrogen sulfide attenuates inflammatory hepcidin by reducing IL-6 secretion and promoting SIRT1-mediated STAT3 deacetylation[Pubmed:26154696] |
| BioMed Research International | The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models.[pubmed:27642598] |
| Frontiers in Neuroscience | In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution.[pubmed:27729845] |
| Experimental Biology and Medicine | Effects of iron supplementation in mice with hypoferremia induced by obesity[pubmed:27439539] |
| Nutrition, Metabolism and Cardiovascular Diseases | Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case–control study[pubmed:27139516] |
| PLOS ONE | S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway.[pubmed:27649298] |
| Endocrine connections | Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expression[pubmed:28179377] |
| Maternal and cord blood hepcidin levels based on gestational weeks in term and preterm infants[publication:305921433_Maternal_and_cord_blood_hepc] | |
| Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation[pubmed:29147463] |
| The Royal Society of Chemistry | Silkworm feces extract improves iron deficiency anemia via suppressing hepcidin expression and promoting iron-regulatory proteins expression[DOI: 10.1039/C7RA09576G] |
| Journal of Inflammation | Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients[10.1186:s12950-017-0166-3] |
| Antioxidants and Redox Signaling | AMPK serves as a Therapeutic Target against Anemia of Inflammation[pubmed:27923278] |
| NutriciónHospitalaria | Micronutrient supplementation in gastric bypass surgery: prospective study on infl ammation and iron metabolism in premenopausal women[10.20960/nh.1162] |
| The Japanese Society of Hematology | Hepcidin suppression in β‑thalassemia is associated with the down‑regulation of atonal homolog 8[pubmed:28405918] |
| Tuberculosis | Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity[10.1016/j.tube.2017.11.011] |
| Oman Medical Journal | The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus.[pubmed:28584599] |
| Cellular Physiology and Biochemistry | The Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing Mice[Pubmed:29843136] |
| Cellular Microbiology | inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages[Pubmed:29470856] |
| Journal of Histochemistry & Cytochemistry | Acute and Chronic Iron Overloading Differentially Modulates the Expression of Cellular Iron-homeostatic Molecules in Normal Rat Kidney[Pubmed:29873589] |
| Clinical and Experimental Medicine | Increased NGAL level associated with iron store in chronic kidney disease with anemia[Pubmed:29909502] |
| Hematology | Relationship between obesity and iron deficiency anemia: is there a role of hepcidin?[Pubmed:29318945] |
| Hepcidin serum levels in HCV chronically mono-infected naïve patients, compared to healthy individuals[77856] | |
| Food & Function | Effect of iron supplement from microalgae on the treatment of iron-deficient anemia in rats[Doi: 10.1039/C8FO01696H] |
| Haematologica | New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia[Pubmed: 30792208] |
| Ann Intensive Care | Inflammatory anemia-associated parameters are related to 28-day mortality in patients with sepsis admitted to the ICU: a preliminary observational study[Pubmed: 31183575] |
| Journal of Functional Foods | Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats[] |
| Naunyn Schmiedebergs Arch Pharmacol. | 3-Amidinophenylalanine-derived matriptase inhibitors can modulate hepcidin production in vitro[Pubmed: 31659405] |
| DEVELOPMENT GROWTH & DIFFERENTIATION | H2O2 induces oxidative stress damage through the BMP‐6/SMAD/hepcidin axis[Pubmed: 32012242] |
| Frontiers in Physiology | Increased Hepcidin Levels During a Period of High Training Load Do Not Alter Iron Status in Male Elite Junior Rowers[Pubmed: 32038278] |
| J Korean Med Sci | Can Calprotectin Show Subclinical Inflammation in Familial Mediterranean Fever Patients?[Pubmed: 32174062] |
| nephrology dialysis transplantation | Iron kinetics following treatment with sucroferric oxyhydroxide or ferric citrate in healthy rats and models of anaemia, iron overload or inflammation[Pubmed: 32259248] |
| mikrochimica acta | Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidin[Pubmed: 33095336] |
| JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in …[Pubmed: 33065276] |
| Sleep Breath | Relation of serum hepcidin levels and restless legs syndrome in chronic hemodialysis patients[Pubmed: 33029690] |
| journal of trace elements in experimental medicine | Difference in iron metabolism may partly explain sex-related variability in the manifestation of Wilson's disease[Pubmed: 32937238] |
| reproduction in domestic animals | Expression of iron regulatory proteins in full term swine placenta[Pubmed: 32449967] |
| Neurobiology of Aging | Estrogen deficiency is associated with brain iron deposition via upregulation of hepcidin expression in aged female mice[Pubmed: 32920472] |
| The Egyptian Journal of Haematology | Urinary hepcidin concentration in assessment of iron homeostasis in pediatrics[] |
| COMPARATIVE MONITORING OF THE ENZYME ACTIVITY OF BONE METABOLISM IN PATIENTS AFTER DENTAL IMPLANTATION[] | |
| Serum iron, prealbumin, D-dimer and prothrombin time are indicators of the diagnosis, severity and outcome of Salmonella enterica serovar Enteritidis infection[] | |
| Гепсидин и индекс фиброза печени MELD-XI как маркеры полиорганной недостаточности у пациентов хронической сердечной недостаточностью с …[] | |
| Ассоциации уровней NT-proBNP и гепсидина с клинико-лабораторными параметрами у больных хронической сердечной недостаточностью с разной …[] | |
| Biosci Rep | Correlation of hepcidin and serum ferritin levels in thalassemia patients at Chiang Mai University Hospital[33565577] |
| Biometals | Iron metabolism is disturbed and anti-copper treatment improves but does not normalize iron metabolism in Wilson's disease[33555495] |
| J Mol Cell Cardiol | Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure[34139233] |
| Continuous Veno-Venous Hemofiltration (CVVH) Improves Iron Metabolism Disorders in Patients With Sepsis: A Single-Center Prospective Cohort Study[] | |
| Reports of Biochemistry & Molecular Biology | Evaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization[] |
| Food Funct | High-dose ferric citrate supplementation attenuates omega-3 polyunsaturated fatty acid biosynthesis via downregulating delta 5 and 6 desaturases in rats with high-fat …[34787162] |
| J Trace Elem Med Biol | Caffeine and alcohol-friends or foes of human iron stores?[Pubmed:35063815] |
| PeerJ | Resveratrol ameliorates iron overload induced liver fibrosis in mice by regulating iron homeostasis[Pubmed:35698613] |
| Serum Calprotectin and Iron Metabolism Biomarker Levels in Behçet's Disease[] | |
| Food Bioscience | Roles of homopolymeric apoferritin in alleviating alcohol-induced liver injury[] |
