γ-氨基丁酸(gABA)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Gamma-Aminobutyric Acid (gABA)
4-Aminobutyric Acid
- 编号CEA900Ge
- 物种Pan-species (General，通用)相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围4.94-400pg/mL
- 灵敏度最小可检测剂量小于等于2.17pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 2827¥ 4039¥ 18176¥ 34333¥ 282744
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产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测γ-氨基丁酸(gABA)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的γ-氨基丁酸(gABA)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 91-99 | 95 |
| EDTA plasma(n=5) | 79-101 | 96 |
| heparin plasma(n=5) | 99-105 | 102 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的γ-氨基丁酸(gABA)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中γ-氨基丁酸(gABA)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-104% | 78-95% | 79-98% | 94-103% |
| EDTA plasma(n=5) | 87-95% | 90-98% | 80-91% | 80-95% |
| heparin plasma(n=5) | 83-102% | 91-98% | 95-102% | 95-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将γ-氨基丁酸(gABA)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA900Ge11 | γ-氨基丁酸(gABA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge21 | γ-氨基丁酸(gABA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge41 | γ-氨基丁酸(gABA)人血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA900Ge01 | γ-氨基丁酸(gABA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| PAA900Ge02 | γ-氨基丁酸(gABA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA900Ge71 | γ-氨基丁酸(gABA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA900Ge81 | γ-氨基丁酸(gABA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| CEA900Ge | γ-氨基丁酸(gABA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA900Ge | 抗γ-氨基丁酸抗体(Anti-gABA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA900Ge | γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA900Ge11 | γ-氨基丁酸(gABA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Toxicology and Industrial Health | Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum[PubMed: 21444355] |
| Neurosci Lett | Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear.[PubMed: 18577424] |
| Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism[PubMed: PMC3623607] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children[PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)[PubMed: PMC3762604] |
| Scientific Reports | Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension[pubmed:27917928] |
| Journal of Neuroscience | Sex differences in the glutamate signaling pathway in juvenile rats[Pubmed:28861894] |
| Scientific Reports | A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme[Pubmed: 30341396] |
| Neurochemical Research | Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission[Pubmed: 30666488] |
| Neuroscience Letters | Differentiation of human glioblastoma U87 cells into cholinergic neuron[Pubmed: 30928478] |
| Neurochemical Journal | Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism[] |
| Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats.[] | |
| Experimental and Therapeutic Medicine | Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsy[Pubmed: 33101467] |
| Exp Anim | Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation[33828024] |
| Neurogenetics | Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases[34101060] |
| Sci Rep | Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission[34475508] |
| J Food Biochem | Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies[Pubmed:35102569] |
