肾酶(RNLS)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Renalase (RNLS)
MAO-C; C10orf59; FAD-Dependent Amine Oxidase; Monoamine oxidase-C; alpha-NAD(P)H oxidase/anomerase
产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测肾酶(RNLS)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的肾酶(RNLS)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 84-93 | 88 |
| EDTA plasma(n=5) | 78-101 | 97 |
| heparin plasma(n=5) | 82-96 | 91 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的肾酶(RNLS)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中肾酶(RNLS)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-99% | 95-103% | 81-97% | 85-99% |
| EDTA plasma(n=5) | 95-104% | 87-95% | 88-101% | 80-101% |
| heparin plasma(n=5) | 79-90% | 78-95% | 92-101% | 78-91% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将肾酶(RNLS)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的肾酶(RNLS)与连接于固相载体上的抗体结合，然后加入生物素化的肾酶(RNLS)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的肾酶(RNLS)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC845Hu02 | 肾酶(RNLS)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC845Hu01 | 肾酶(RNLS)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC845Hu03 | 肾酶(RNLS)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC845Hu01 | 肾酶(RNLS)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC845Hu02 | 肾酶(RNLS)多克隆抗体 | WB; IHC; ICC; IP. |
| LAC845Hu71 | 肾酶(RNLS)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAC845Hu25 | 肾酶(RNLS)单克隆抗体 | WB |
| MAC845Hu22 | 肾酶(RNLS)单克隆抗体 | WB |
| MAC845Hu21 | 肾酶(RNLS)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC845Hu23 | 肾酶(RNLS)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC845Hu | 肾酶(RNLS)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC845Hu | 肾酶(RNLS)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Pol Arch Med Wewn. | Renalase, kidney function, and markers of endothelial dysfunction in renal transplant recipients[Cartelcolor: source] |
| Angiology | Renalase, Stroke, and Hypertension in Hemodialyzed Patients[PubMed: 22583169] |
| Peritoneal dialysis international | Renalase in peritoneal dialysis patients is not related to blood pressure, but to dialysis vintage.[Pdiconnect: Source] |
| Archives of Medical Science | Vascular adhesion protein-1 and renalase in regard to diabetes in hemodialysis patients[PubMed: PMC3542495] |
| Renal Failure | Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients[Pubmed: 23530612] |
| Borgis - Postepy Nauk Medycznych | VAP-1 in chronic kidney disease and diabetic kidney disease[Czytelniamedyczna: Source] |
| Journal of Basic and Clinical Physiology and Pharmacology | The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy[Pubmed: 15365253] |
| Exp Biol Med (Maywood). | Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients.[Pubmed: 24599883] |
| Kidney Blood Press Res. | Renalase, a novel enzyme involved in blood pressure regulation, is related to kidney function but not to blood pressure in hemodialysis patients.[Pubmed: 22539018] |
| Nephrology Dialysis Transplantation | Renalase in hypertension and kidney disease[Oxfordjournals: Source] |
| Lipids in Health and Disease | Markers of increased cardiovascular risk in patients with chronic kidney disease[Biomedcentral:Source] |
| Research & Experimental? | Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients[Pubmed:24599883] |
| Pediatr Nephrol | Normal values for urine renalase excretion in children[Pubmed:25060760] |
| J Am Soc Hypertens. | Low plasma renalase concentration in hypertensive patients after surgical repair of coarctation of aorta[Pubmed:25064768] |
| Cardiology. | Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults[Pubmed:25824645] |
| J Am Soc Hypertens | Circulating renalase, catecholamines, and vascular adhesion[PubMed: 26403854] |
| Indian Pediatr | Renalase levels in children with solitary functioning kidney[PubMed: 26713989] |
| Oxford Journals | SP321 RENALASE, RENAL FUNCTION AND BIOMARKERS OF ENDOTHELIAL DYSFUNCTION IN CHRONIC KIDNEY DISEASE PATIENTS[Content: 30] |
| journal of hypertension | PP.27.07: PREHYPERTENSIVE ALTERATIONS OF ARTERIAL AND VENOUS REACTIVITY IN A RAT POLYCYSTIC OVARY SYNDROME MODEL. EFFECTS OF PARALLEL VITAMIN D3 ADMINISTRATION[Jhypertension: Abstract] |
| journal of hypertension | PP.27.06: RENALASE, RENAL FUNCTION AND BIOMARKERS OF ENDOTHELIAL DYSFUNCTION IN CHRONIC KIDNEY DISEASE PATIENTS.[Jhypertension: Abstract] |
| journal of hypertension | PP.27.04: ENDOTHELIAL DYSFUNCTION AND ARTERIAL HYPERTENSION IN THE WORKING POPULATION (MERIDIAN STUDY).[Jhypertension: Abstract] |
| Curr Vasc Pharmacol. | Renalase is Removed by Kidneys and During Dialysis – Excess Related to CKD Complications?[PubMed: 25515790] |
| Kidney Blood Press Res | Effects of Renin-Angiotensin System Inhibitors on Renal Expression of Renalase in Sprague-Dawley Rats Fed With High Salt Diet[PubMed: 26619289] |
| PLoS One | Serum Renalase Levels Correlate with Disease Activity in Lupus Nephritis[PubMed: 26431044] |
| J Hum Hypertens | Plasma renalase concentration before and after radiofrequency renal denervation in patients with resistant hypertension[PubMed: 26310184] |
| Nephrology (Carlton) | Urinary renalase concentration in patients with preserved kidney function undergoing coronary angiography.[pubmed:27778420] |
| Nephrol Dial Transplant | PLASMA RENALASE CONCENTRATION BEFORE AND AFTER RADIOFREQUENCY RENAL DENERVATION IN PATIENTS WITH RESISTANT HYPERTENSION: PRELIMINARY RESULTS[content:31] |
| Renal Failure | Increased serum renalase in hemodialysis patients: is it related to left ventricular hypertrophy?[pubmed:27416751] |
| Enhanced biocompatible and hemocompatible nano/micro porous surface as a biological scaffold for functionalizational and biointegrated implants | High-performance Liquid Chromatography Measured Metabolites of EndogenousCatecholamines and Their Relations to Chronic Kidney Disease and High Blood Pressure in Heart Transplant Recipients.[pubmed:27496485] |
| PLOS ONE | Online Haemodiafiltration Improves Inflammatory State in Dialysis Patients: A Longitudinal Study.[pubmed:27783636] |
| Advances in Experimental Medicine and Biology | Renalase in Children with Glomerular Kidney Diseases[chapter:10.1007] |
| Kidney & Blood Pressure Research | Plasma Renalase is Not Associated with Blood Pressure and Brachial-Ankle Pulse Wave Velocity in Chinese Adults With Normal Renal Function[452587] |
| BMC nephrology | Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controls[pubmed:28372594] |
| Nefrología (English Edition) | Increased serum renalase in peritoneal dialysis patients: Is it related to cardiovascular disease risk[pubmed:28160962] |
| Clinical Interventions in Aging | Age influence on renalase and catecholamines concentration in hypertensive patients, including maintained dialysis[pubmed:27822026] |
| International Journal of Clinical and Experimental Medicine | Aumento de renalasa sérica en pacientes de diálisis peritoneal:¿ está relacionado con el riesgo de enfermedad cardiovascular?[10.1016:j.nefro.2016.11.013] |
| PLoS One | Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients[pubmed:28614373] |
| Annals of Agricultural and Environmental Medicine | Levels of renalase and advanced oxidation protein products with regard to catecholamines in haemodialysed patients[pubmed:28954489] |
| Angiology | Endocan Concentration in Patients With Primary Hypertension[pubmed:29084442] |
| Journal of Hypertension | RENALASE PLASMA CONCENTRATION BEFORE AND AFTER RADIOFREQUENCY RENAL DENERVATION IN PATIENTS WITH RESISTANT HYPERTENSION[10.1097/01.hjh.0000523893.07419.ae] |
| International journal of molecular sciences | Renalase Protects against Renal Fibrosis by Inhibiting the Activation of the ERK Signaling Pathways[pubmed:28448446] |
| Journal of sports medicine and physical fitness | Transient changes in serum renalase concentration during long-distance running: The case of an amateur runner under continuous training[10.7600/jpfsm.6.159] |
| Scientific Reports | Synergistic effect of renalase and chronic kidney disease on endothelin-1 in patients with coronary artery disease‒a cross-sectional study[Pubmed:29743680] |
| Life Sciences | Influence of acute exercise on renalase and its regulatory mechanism[Pubmed:30056018] |
| Experimental Eye Research | Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration[Pubmed:29580721] |
| Rapid Communications in Mass Spectrometry | A new method for quantitative determination of renalase based on mass‐spectrometry determination of a proteotypic peptide labeled with stable isotopes[10.1002:rcm.8167] |
| International Urology and Nephrology | Renalase contributes to protection against renal fibrosis via inhibiting oxidative stress in rats[Pubmed:29476431] |
| Pediatric Cardiology | Serum Renalase Levels in Adolescents with Primary Hypertension[Pubmed:29748702] |
| Postepy w Kardiologii Interwencyjnej | Atorvastatin facilitates protection against contrast-induced nephropathy in patients undergoing coronary angiography via humoral mediators rather than …[Pubmed:30008773] |
| High urinary kidney injury molecule type 1 level is associated with 12-month adverse cardiovascular events in patients with coronary artery disease[publication:324522368] | |
| Journal of Clinical Medicine | Serum Renalase Levels Are Predicted by Brain-Derived Neurotrophic Factor and Associated with Cardiovascular Events and Mortality after Percutaneous Coronary …[Pubmed: 30424498] |
| Archives of disease in childhood | GP23 Percutaneous closure of patent ductus arteriosus: single center experience[] |
| Archives of disease in childhood | GP25 Comparison of associations and outcomes of prenatally and postnatally diagnosed right aortic arch: south wales' experience[] |
| Archives of disease in childhood | GP24 Serum renalase levels in adolescents with primary hypertension[] |
| Disease Markers | Association of Plasma Renalase and Left Ventricle Mass Index in Heart Failure Patients Stratified to the Category of the Ejection Fraction: A Pilot Study[Pubmed: 31737132] |
| EXPERIMENTAL BIOLOGY AND MEDICINE | The role of urinary renalase on early-stage renal damage in Chinese adults with primary hypertension[Pubmed: 32102561] |
| FEBS Open Bio | Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats[Pubmed: 32053739] |
| current medical research and opinion | The partnership between renalase and ejection fraction as a risk factor for increased cardiac remodeling biomarkers in chronic heart failure patients[Pubmed: 32297799] |
| Dovepress | Association of Serum Renalase Levels and Renalase rs10887800 Polymorphism with Unstable Angina Pectoris Patients Having Metabolic Syndrome[Pubmed: 32982355] |
| 学位论文 | Nikotinamidadenindinukleotid-Blutplasmawerte sind unabhängig vom kalendarischen und vaskulären Alter in einer Normalkohorte aus der Region Halle-Saalekreis[] |
| Life | Circulating Renalase as Predictor of Renal and Cardiovascular Outcomes in Pre-Dialysis CKD Patients: A 5-Year Prospective Cohort Study[33800219] |
| Edited by: Background: Renalase has been implicated in chronic heart failure (CHF); however, nothing is known about renalase discriminatory ability and prognostic …[] |
