武汉云克隆科技股份有限公司

分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)

SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b

  • 分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法)实验结果图
  • SEC842Hu.jpg标准曲线图
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测分泌型卷曲相关蛋白5(SFRP5)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的分泌型卷曲相关蛋白5(SFRP5)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)79-9383
EDTA plasma(n=5)85-9288
heparin plasma(n=5)87-10598

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的分泌型卷曲相关蛋白5(SFRP5)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中分泌型卷曲相关蛋白5(SFRP5)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)93-101%98-105%90-104%80-92%
EDTA plasma(n=5)93-101%94-102%78-99%86-94%
heparin plasma(n=5)98-105%83-92%81-93%89-97%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将分泌型卷曲相关蛋白5(SFRP5)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的分泌型卷曲相关蛋白5(SFRP5)与连接于固相载体上的抗体结合，然后加入生物素化的分泌型卷曲相关蛋白5(SFRP5)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的分泌型卷曲相关蛋白5(SFRP5)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPC842Hu01分泌型卷曲相关蛋白5(SFRP5)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPC842Hu02分泌型卷曲相关蛋白5(SFRP5)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPC842Hu03分泌型卷曲相关蛋白5(SFRP5)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAC842Hu01分泌型卷曲相关蛋白5(SFRP5)多克隆抗体WB; IHC; ICC; IP.
PAC842Hu03分泌型卷曲相关蛋白5(SFRP5)多克隆抗体WB; IHC; ICC; IP.
PAC842Hu02分泌型卷曲相关蛋白5(SFRP5)多克隆抗体WB
LAC842Hu71分泌型卷曲相关蛋白5(SFRP5)多克隆抗体(生物素标记)WB; IHC; ICC.
MAC842Hu22分泌型卷曲相关蛋白5(SFRP5)单克隆抗体WB; IHC; ICC; IP.
LAC842Hu72分泌型卷曲相关蛋白5(SFRP5)单克隆抗体(生物素标记)WB; IHC; ICC.
SEC842Hu分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
WEC842Hu分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法,宽范围)Enzyme-linked immunosorbent assay for Antigen Detection.
HEC842Hu分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法,高敏型)Enzyme-linked immunosorbent assay for Antigen Detection.
LMC842Hu分泌型卷曲相关蛋白5(SFRP5)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
Diabetes Research and Clinical PracticePlasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance[PubMed: 23290274]
European Journal of Clinical InvestigationSfrp5 correlates with insulin resistance and oxidative stress[PubMed: 23398169]
Pediatric ResearchBalanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children[Pubmed: 24603290]
Clin Endocrinol (Oxf).SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children[Pubmed: 24330025]
The Journal of Clinical Endocrinology & MetabolismCirculating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humans[Pubmed: 23185036]
AtherosclerosisLow serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease[Pubmed: 24530778]
PLoS One.Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation[Pubmed:Pmc4072672]
J Clin Endocrinol Metab.Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation[Pubmed:24840810]
Oncology LettersSerum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma[Pubmed:25120720]
Diabetes Metab Res Rev.Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls[Pubmed:25139699]
Clinical &amp; Experimental ImmunologyThe wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis[Pubmed:25382802]
Diabetes Metab Res RevCirculating SFRP5 levels are elevated in drug-na[PubMed: 25139699]
J Matern Fetal Neonatal MedPrediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels[PubMed: 26100762]
Eur J Clin InvestSfrp5 associates with beta-cell function in humans[Pubmed:27019073]
Osteoporosis InternationalBone marrow sFRP5 level is negatively associated with bone formation markers[pubmed:27986984]
Diabetes/Metabolism Research and ReviewsPlasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults[pubmed:28303637]
Cardiovascular DiabetologyInverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study[pubmed:28851362]
Arteriosclerosis, Thrombosis, and Vascular Biology Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With …[Pubmed:29674475]
ObesityLongitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans[Pubmed:29427376]
J Bas Res Med SciDecreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats[article-1-352-en.pdf]
Molecular Biology ReportsThe effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndrome[Pubmed: 30194556]
Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling[]
British Journal Of NutritionA diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile[Pubmed: 30375290]
J Clin Endocrinol MetabRole of wnt5a in Metabolic Inflammation in Humans[Pubmed: 30137542]
Frontiers in EndocrinologyAssociation of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients[Pubmed: 32300333]
CLINICAL BIOCHEMISTRYSfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease[Pubmed: 33188773]
Sao Paulo Medical JournalAssociation of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and …[Pubmed: 32491086]
Kidney Blood Press ResSecreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway[34469882]
CytokineAssociation of serum uromodulin with adipokines in dependence of type 2 diabetes[34920231]
PLoS OneAssociation of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study[Pubmed:34990470]
JMIR Res ProtocInvestigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition …[Pubmed:35475790]
DiabetologyEarly Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop …[]