巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Macrophage Migration Inhibitory Factor (MIF)
GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase
- 编号SEA698Po
- 物种Sus scrofa; Porcine (Pig，猪)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.057ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 3175¥ 4536¥ 20412¥ 38556¥ 317520
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特异性
本试剂盒用于检测巨噬细胞移动抑制因子(MIF)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的巨噬细胞移动抑制因子(MIF)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 82-102 | 95 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 93-101 | 97 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的巨噬细胞移动抑制因子(MIF)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中巨噬细胞移动抑制因子(MIF)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-104% | 85-104% | 79-88% | 90-97% |
| EDTA plasma(n=5) | 81-94% | 82-96% | 97-105% | 95-102% |
| heparin plasma(n=5) | 93-101% | 82-99% | 95-102% | 89-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将巨噬细胞移动抑制因子(MIF)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的巨噬细胞移动抑制因子(MIF)与连接于固相载体上的抗体结合，然后加入生物素化的巨噬细胞移动抑制因子(MIF)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的巨噬细胞移动抑制因子(MIF)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| SEA698Po | 巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA698Po | 巨噬细胞移动抑制因子(MIF)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Kidney International | Targeted reduction of advanced glycation improves renal function in obesity[PubMed: 21412218] |
| The Journal of Immunology | Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut[Jimmunol: 5673] |
| The Journal of Endocrinology | Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease[PubMed: PMC3757527] |
| The Journal of Immunology | Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice[PubMed: PMC3466372] |
| Diabetologia | Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes[Pubmed:24957662] |
| BioMed Research International | The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder[PubMed: 25705692] |
| Diabetologia | Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease.[pubmed:28160092] |
| Life Sciences | Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis [pubmed:28919396] |
| Scientific Reports | Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke[Pubmed:29335619] |
| Ocular Immunology and Inflammation | Immune Response and Mechanisms of IFN-γ in Administration for Keratomycosis[Pubmed: 30307777] |
| International Journal of Immunopathology and Pharmacology | Effect of voluntary running on expression of myokines in brains of rats with depression[Pubmed: 30834799] |
| Biomarkers in Heart Failure and Associated Diseases | Biomarkers in Heart Failure and Associated Diseases[] |
| Science China-Life Sciences | Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation[Pubmed: 31209799] |
| Folia Morphologica | Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression[Pubmed: 31802473] |
| Biomed Pharmacother | Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis[Pubmed: 32062385] |
| Sci Rep | Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population[33850223] |
| Commun Biol | Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes[Pubmed:35115654] |
