武汉云克隆科技股份有限公司

基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Matrix Gla Protein (MGP)

MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

  • 基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法)实验结果图
  • SEB477Mu.jpg标准曲线图
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测基质Gla蛋白(MGP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的基质Gla蛋白(MGP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)95-10399
EDTA plasma(n=5)83-10398
heparin plasma(n=5)99-105102

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的基质Gla蛋白(MGP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中基质Gla蛋白(MGP)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)94-101%80-91%84-93%91-105%
EDTA plasma(n=5)87-97%80-90%78-103%78-95%
heparin plasma(n=5)83-101%78-98%78-101%87-95%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将基质Gla蛋白(MGP)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的基质Gla蛋白(MGP)与连接于固相载体上的抗体结合，然后加入生物素化的基质Gla蛋白(MGP)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质Gla蛋白(MGP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号适用物种：Mus musculus (Mouse，小鼠)应用(仅供研究使用，不用于临床诊断！)
RPB477Mu02基质Gla蛋白(MGP)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB477Mu01基质Gla蛋白(MGP)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB477Mu01基质Gla蛋白(MGP)多克隆抗体WB; IHC; ICC; IP.
PAB477Mu02基质Gla蛋白(MGP)多克隆抗体WB; IHC; ICC; IP.
LAB477Mu71基质Gla蛋白(MGP)多克隆抗体(生物素标记)WB; IHC; ICC.
SEB477Mu基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB477Mu基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
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Maturitas. Bone metabolism regulators and arterial stiffness in postmenopausal women[Pubmed: 23916080]
The Egyptian RheumatologistOsteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activity[ScienceDirect: S1110116414000076]
Toxicology ReportsCanola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice[Researchgate:Source]
Lipids in Health and DiseaseMarkers of increased cardiovascular risk in patients with chronic kidney disease[Biomedcentral:Source]
Human &amp; Veterinary MedicineThe behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veins[Hvm:Source]
Pol Arch Med WewnEffect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5[PubMed: 26176325]
Lipids Health Dis.Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary study[pmc:PMC4739105]
Kidney Blood Press Res.Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney Disease[Pubmed:27100101]
Archives of Oral BiologyMatrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculus[pubmed:27348051]
Cell Death & DiseaseMesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis[Pubmed:29880866]
UrologyHigh concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla protein[Pubmed:29935264]
Clinica Chimica ActaSerum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseases[Pubmed: 30597137]
Biological Trace Element ResearchAltered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients[Pubmed: 30847765]
Renal FailureRelationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patients[Pubmed: 31538831]
HeliyonMatrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4[Pubmed: 32322728]