Ⅱ型胶原(COL2)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Collagen Type II (COL2)
- 编号SEA572Mu
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.114ng/mL.
- 样本类型serum, plasma, tissue homogenates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 2722¥ 3888¥ 17496¥ 33048¥ 272160
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特异性
本试剂盒用于检测Ⅱ型胶原(COL2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Ⅱ型胶原(COL2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 79-99 | 93 |
| EDTA plasma(n=5) | 89-97 | 93 |
| heparin plasma(n=5) | 83-98 | 87 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Ⅱ型胶原(COL2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅱ型胶原(COL2)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-98% | 78-102% | 85-97% | 90-99% |
| EDTA plasma(n=5) | 97-105% | 95-103% | 82-93% | 95-104% |
| heparin plasma(n=5) | 90-103% | 87-97% | 92-101% | 93-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将Ⅱ型胶原(COL2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的Ⅱ型胶原(COL2)与连接于固相载体上的抗体结合，然后加入生物素化的Ⅱ型胶原(COL2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Ⅱ型胶原(COL2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| PAA572Mu01 | Ⅱ型胶原(COL2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA572Mu71 | Ⅱ型胶原(COL2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA572Mu | Ⅱ型胶原(COL2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA572Mu | Ⅱ型胶原(COL2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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